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H Kim, SC Park, Y Kim, J Suh; Effect of Antisense against c-met/Hepatocyte Growth Factor receptor . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4627.
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Purpose: To determine the effect of phosphorothioate antisense oligonucletides directed against c-met/HGF receptor on RPE cell differentiation and proliferation. Methods: We transfected cultured human RPE with c-met-specific phosphorothioate antisense oligonucleotides that were designed as 18-mer sequences complementary to c-met mRNA. Target mRNA was quantified by RNase protection assay. Cellular differentiation and proliferation were determined by nitro blue tetrazolium (NBT) dye reduction and immunocytochemistry of CD44 RPE cell surface antigen expression. Results: Transfection of c-met antisense oligonucleotides (2uM) reduced the expression of c-met mRNA by 50% to 65%. It also inhibited RPE cell proliferation and blocked CD44 expression. Conclusion: Antisense oligonucleotides effectively inhibit c-met expression, RPE cell differentiation and proliferation.
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