December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Selective Uptake-mechanisms in Rabbit Retinal Cells
Author Affiliations & Notes
  • K-H Huemer
    Dept of Physiology Univ of Vienna Vienna Austria
  • C Simader
    Dept Ophthalmology Univ of Vienna / General Hospital Vienna Austria
  • T Barisani-Asenbauer
    Dept Ophthalmology Univ of Vienna / General Hospital Vienna Austria
  • Footnotes
    Commercial Relationships   K. Huemer, None; C. Simader, None; T. Barisani-Asenbauer, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4756. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K-H Huemer, C Simader, T Barisani-Asenbauer; Selective Uptake-mechanisms in Rabbit Retinal Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4756.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: The labeling of D1 receptors by the selective D1 antagonist BODIPY-FL SCH23390 (Molecular Probes) can be recognized by fluorescence restricted to cell surfaces (see e.g. Huemer et al ARVO 2000). But in several types of retinal cells an uptake can be shown by cytosolic distribution of the substance in confocal microscopy. The characterization of putative uptake mechanisms is purpose of this study. Methods: Rabbit retinae were isolated immediately after sacrification and transferred to cooled culture medium. The fluorescent labeled D1 antagonist BODIPY-FL SCH23390 and different uptake inhibitors were added to the medium and retinae incubated for 15 to 120 min. Then retinae were transferred to a covered chamber with the ganglion cell layer facing down and image series were taken with an inverted laser scanning confocal microscope (Argon Laser 488nm, EM 505nm) Results: BODIPY-FL SCH23390 is distributed within the cytosol of Müller cells and in a population of retinal ganglion cells. An uptake into bipolar cells is implied by our earlier study but outside the confocal focus range in whole mounts and hence could not be further examined in this study. In ganglion cells we found a dose dependent uptake inhibition of this uptake by desipramin, but not by cocaine or nomifensin. Conclusion: We can show that the use of substances like BODIPY-FL SCH23390 can indicate local uptake in addition to receptor labelling. We suggest a mechanism related to indoleamine uptake for BODIPY-FL SCH23390 as indicated by the desipramine sensitivity. A relationship to mechanisms for the in vivo uptake of transmitters is likely but has yet to be examined further.

Keywords: 554 retina • 490 neurotransmitters/neurotransmitter systems 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.