December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Effects of Acetylcholine and Its Analogs on Intracellular Calcium Level in the Regenerating Retina
Author Affiliations & Notes
  • M Ohmasa
    Institute of Biological Sciences University of Tsukuba Tsukuba Japan
  • T Saito
    Institute of Biological Sciences University of Tsukuba Tsukuba Japan
  • Footnotes
    Commercial Relationships   M. Ohmasa, None; T. Saito, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4759. doi:
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      M Ohmasa, T Saito; Effects of Acetylcholine and Its Analogs on Intracellular Calcium Level in the Regenerating Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4759.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Acetylcholine (ACh) has been shown to be involved in many aspects of developmental processes of the CNS through changes in the intracellular Ca2+ ion concentrations ([Ca2+]i) . To investigate whether ACh is also involved in the regenerating processes of adult newt retina, we examined the effects of ACh on [Ca2+]i in the regenerating retina using Ca2+ imaging techniques and the time course of appearance of ACh receptors (AChRs) during regeneration using immunohistochemical techniques. Methods: We incubated living slice preparations of regenerating retinas at various stages with a Ca2+-sensitive fluorescent dye (Fura-2). We calculated the 340nm/380nm fluorescence intensity ratio to estimate relative changes in [Ca2+]i. Regenerating retinas were immunostained using antibodies against ACh receptors. Results: At early stage of retinal regeneration, ACh elevated [Ca2+]i in Fura-2-labeled retinal progenitor cells. The ACh-induced [Ca2+]i rise was mainly mediated by activation of an m1- (rather than m2-) type mAChR. This was also supported by the immunohistochemistry. As regeneration proceeded, the number of ACh-responsive cells and peak amplitude of their responses decreased to control levels. Conclusion: The ACh is involved in early regenerating processes as well as developing processes through m1 mAChR-mediated increase in [Ca2+]i .

Keywords: 564 retinal development • 305 acetylcholine • 334 calcium 
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