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Ko Eun Kim, Inseok Jang, Hyungwon Moon, Yu Jeong Kim, Jin Wook Jeoung, Ki Ho Park, Hyuncheol Kim; Neuroprotective Effects of Human Serum Albumin Nanoparticles Loaded With Brimonidine on Retinal Ganglion Cells in Optic Nerve Crush Model. Invest. Ophthalmol. Vis. Sci. 2015;56(9):5641-5649. doi: 10.1167/iovs.15-16538.
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© ARVO (1962-2015); The Authors (2016-present)
We investigated the neuroprotective effect of human serum albumin nanoparticles (HSA-NPs) and their conjugation with brimonidine (HSA-Br-NPs) on retinal ganglion cells (RGCs) in optic nerve crush (ONC) model.
We fabricated HSA-Br-NPs by ethanol precipitation, including 0.18% brimonidine (Br) and 3.5% human serum albumin (HSA) in HSA-Br-NP solution. We performed ONC and intravitreal injection in Sprague–Dawley rats, which were divided into (1) Normal, (2) balanced salt solution (BSS)–injected ONC, (3) HSA-NP–injected ONC, (4) Br-injected ONC, and (5) HSA-Br-NP–injected ONC groups. Survival of RGC was compared 5 and 14 days after procedures. A cell viability assay evaluated the amyloid-β (Aβ)–associated neuroprotective mechanism of HSA-NP.
The HSA-Br-NPs showed a narrow size distribution (152.8 ± 51.1 nm) and a negatively charged surface (−29.7 ± 7.5 mV), releasing Br for 5 days. The percentages of RGC survival in the HSA-NP (52.6 ± 3.3%), Br (58.0 ± 4.2%), and HSA-Br-NP (63.5 ± 7.1%) groups relative to Normal (100%) were significantly higher than in the BSS group (29.2 ± 3.3%) 5 days after ONC (P < 0.001). However, the HSA-Br-NP (38.1 ± 3.6%) group showed significantly higher RGC density than the BSS (10.3 ± 5.6%, P < 0.001) or Br (18.6 ± 3.9%, P = 0.006) group at 14 days. The HSA-NP injection reduced Aβ deposition in the RGC layer of ONC model, and a cell viability test showed that HSA-NP can inhibit Aβ-induced RGC death.
Human serum albumin nanoparticles showed neuroprotective potential by inhibiting Aβ deposition, and exerted a sustained therapeutic effect with the combined neuroprotective agent. Our results suggest the potential of HSA-Br-NP as a promising neuroprotective agent.
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