September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Sustain release of genistein from nanoparticles surface modified IOL by ester bond to prevent posterior capsular opacification in rabbit eyes
Author Affiliations & Notes
  • Jinlu Liu
    Ophthalmology, The fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
    Ophthalmology, Moran eye center, Salt Lake City, Utah, United States
  • Balamurali Ambati
    Ophthalmology, Moran eye center, Salt Lake City, Utah, United States
  • Jun Kong
    Ophthalmology, The fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
  • Footnotes
    Commercial Relationships   Jinlu Liu, None; Balamurali Ambati, None; Jun Kong, None
  • Footnotes
    Support  National Natural Science Foundation of China (No. 81100654)
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4016. doi:
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    • Get Citation

      Jinlu Liu, Balamurali Ambati, Jun Kong; Sustain release of genistein from nanoparticles surface modified IOL by ester bond to prevent posterior capsular opacification in rabbit eyes. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4016.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Many drugs have been loaded onto IOLs by different methods to achieve a sustained and controlled release of drugs to treat posterior capsular opacification (PCO). This study tested the hypothesis that the sustained release of genistein (GEN) from negative charged nanoparticles surface modified IOL by ester bond can prevent the PCO in rabbit eyes effectively and safely.

Methods : Nanostructured lipid carriers (NLCs) loaded with GEN (GEN-NLC) were made by melt emulsification method. GEN-NLCs were attached on IOLs by ester bond formed by the dehydration between the carboxyl group offered by the sodium deoxycholate on the NLC surface and the hydroxy group of hydrophilic acrylic IOLs. The morphology and release of the GEN-NLC on the surface of IOLs and was measured. Sixty New Zealand albino rabbits undergoing phacoemulsification in left eye were randomly and equally divided into three groups. Group A: original IOL; group B: anterior chamber (AC) injection GEN-NLC and original IOL; group C: GEN-NLC-IOL. The concentrations of GEN in the AC were determined. The degree of PCO was evaluated after 12 weeks. The histological sections, immunohistochemistry expression of proliferating cell nuclear antigen (PCNA) in the lens capsule were conducted. The anterior and posterior tissues were histologically examined by light and electron microscopes. One way ANOVA was used to statistical analysis.

Results : Spherical-shape particles around 150nm were showed on IOLs. It exhibits a burst release of GEN in 6h followed by a constant release up to 72hours with more than 97% drug was released. Significant difference was found between group B and C of the AC GEN concentrations for any time point(F=496.976,P<0.001). There were remarkably less proliferative tissues covering on the posterior capsules in group C than in the other two groups. A significantly weak expression of PCNA in the nuclei of LECs of posterior capsule was seen in group C than other groups(p < 0.001).No ocular noxious damage to the cornea, iris, cliliary body or retina under the light and electron microscope detection.

Conclusions : Ester bond was a promising method for drugs loaded on hydrophilic acrylic IOLs. Intraocular Gen-NLC-IOL implantation was a promising, effective and safe administration system in the rabbit PCO model.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

 

No obvious toxic were seen of GEN-NLC to the corena and the retina in all groups

No obvious toxic were seen of GEN-NLC to the corena and the retina in all groups

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