September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Photodynamic Antimicrobial Therapy to inhibit Purpureocillium lilacinum, Pseudallescheria boydii and Cochliobolus lunatus isolates.
Author Affiliations & Notes
  • Nidhi Relhan
    Ophthalmic Biophysics Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Alejandro Arboleda
    Ophthalmic Biophysics Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Heather Ann Durkee
    Ophthalmic Biophysics Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Mariela C Aguilar
    Ophthalmic Biophysics Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Karam AlRahman Alawa
    Ophthalmic Biophysics Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Cornelis Rowaan
    Ophthalmic Biophysics Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Guillermo Amescua
    Anne Bates Leach Eye Hospital, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Darlene Miller
    Ocular Microbiology Laboratory, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Harry W Flynn
    Anne Bates Leach Eye Hospital, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Jean-Marie A Parel
    Ophthalmic Biophysics Center, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    CHU Sart-Tillman, Department of Ophthalmology, University of Liege, Liege, Belgium
  • Footnotes
    Commercial Relationships   Nidhi Relhan, None; Alejandro Arboleda, None; Heather Durkee, None; Mariela Aguilar, None; Karam Alawa, None; Cornelis Rowaan, None; Guillermo Amescua, None; Darlene Miller, None; Harry Flynn, None; Jean-Marie Parel, None
  • Footnotes
    Support  Supported in part by the Florida Lions Eye Bank, Edward D. and Janet K. Robson Foundation, Drs. KR Olsen and ME Hildebrandt, Dr. Raksha Urs and Aaron Furtado, NIH center grant P30EY14801, Research to Prevent Blindness, and the Henri and Flore Lesieur Foundation (JMP). The authors are grateful to Alex Gonzalez, Juan Silgado, Victor Hernandez, and Adriana Henao-Pink for their technical contributions.
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2343. doi:
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      Nidhi Relhan, Alejandro Arboleda, Heather Ann Durkee, Mariela C Aguilar, Karam AlRahman Alawa, Cornelis Rowaan, Guillermo Amescua, Darlene Miller, Harry W Flynn, Jean-Marie A Parel; Photodynamic Antimicrobial Therapy to inhibit Purpureocillium lilacinum, Pseudallescheria boydii and Cochliobolus lunatus isolates.. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2343.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To assess the use of rose bengal- and riboflavin-mediated photodynamic antimicrobial therapy (PDAT) to inhibit the growth of three fungal isolates that cause infectious keratitis.

Methods : Three fungi, Purpureocillium lilacinum (Paecilomyces lilacinus), Pseudallescheria boydi, Cochliobolus lunatus (Curvularia lunata), were isolated from patients with confirmed fungal keratitis and grown on Sabouraud-Dextrose agar plates. Using the method we published previously (Arboleda et al, AJO 2014 Jul;158(1):64-70), triplicate test plates were separated into 7 groups: (1) control (fungal isolate only), (2) green irradiation only, (3) 0.1% rose bengal only, (4) 0.1% rose bengal-mediated PDAT (rose bengal + green irradiation), (5) UV irradiation only, (6) 0.1 % riboflavin only, and (7) 0.1% riboflavin-mediated PDAT (riboflavin + UV-A irradiation). Irradiation was performed using either a 47mm diameter 518nm green light emitting diode (LED) array or a 37mm diameter 375nm UV-A LED array for a final energy density of 5.4 J/cm2. After treatment, plates were placed in a 30°C non-CO2incubator and observed for growth. Plates were photographed at day 3 to document fungal growth and images were analyzed using a Labview program created in our laboratory.

Results : Rose bengal-mediated PDAT successfully inhibited the growth of Purpureocilium lilacinum and Pseudallescheria boydii. For these two fungi, rose bengal-mediated PDAT showed complete inhibition within the central 47mm area corresponding to the diameter of the light source at day 3. Cochliobolus lunatus showed minimal inhibition in the central region, but no clear inhibition zone. No other groups demonstrated any inhibitory effect on the three fungal isolates.

Conclusions : Rose bengal-mediated PDAT inhibited Purpureocilium lilacinum and Pseudallescheria boydii keratitis isolates within the irradiated area; however it was not effective for Cochliobolus lunatus. Rose bengal-mediated PDAT has the potential to be a treatment option for fungal keratitis.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

Inhibition of three fungal species: (Row 1) Control, (Row 2) Green Irradiation Only, (Row 3) 0.1% Rose Bengal Only, (Row 4) 0.1% Rose bengal-mediated PDAT.

Inhibition of three fungal species: (Row 1) Control, (Row 2) Green Irradiation Only, (Row 3) 0.1% Rose Bengal Only, (Row 4) 0.1% Rose bengal-mediated PDAT.

 

Inhibition of three fungal species: (Row 1) Control, (Row 2) UV-A Irradiation Only, (Row 3) 0.1% Riboflavin Only, (Row 4) 0.1% Riboflavin-mediated PDAT.

Inhibition of three fungal species: (Row 1) Control, (Row 2) UV-A Irradiation Only, (Row 3) 0.1% Riboflavin Only, (Row 4) 0.1% Riboflavin-mediated PDAT.

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