September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Abnormal anterior lens epithelium in retinitis pigmentosa-related cataract – scanning and transmission electron microscopy study

Hawlina M, MD, PhD 1, Drašlar K, PhD 2, Hvala A, PhD 3, Andjelic S, PhD1

1Eye Hospital, University Medical Centre, Ljubljana, Slovenia
2Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
3Department of Pathology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia
Author Affiliations & Notes
  • Marko Hawlina
    Eye Hospital, University Medical Centre Ljubljana, Ljubljana, Slovenia
  • Footnotes
    Commercial Relationships   Marko Hawlina, None
  • Footnotes
    Support  ARRS P3-0333
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2513. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Marko Hawlina; Abnormal anterior lens epithelium in retinitis pigmentosa-related cataract – scanning and transmission electron microscopy study

      Hawlina M, MD, PhD 1, Drašlar K, PhD 2, Hvala A, PhD 3, Andjelic S, PhD1

      1Eye Hospital, University Medical Centre, Ljubljana, Slovenia
      2Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
      3Department of Pathology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2513.

      Download citation file:


      © 2017 Association for Research in Vision and Ophthalmology.

      ×
  • Supplements
Abstract

Purpose : In retinitis pigmentosa (RP) patients, lens opacity usually develops in the posterior pole and may cause disproportionate functional symptoms requiring cataract operation. The reason of development of this peculiar type of cataract is at present unknown. To investigate possible structural reasons, we studied the ultrastructure of the lens epithelium of RP patients.

Methods : Seven anterior lens capsules from five patients patients with sporadic RP (aLC: basement membrane and associated lens epithelial cells, LECs) were obtained from cataract surgery and prepared for both scanning and transmission electron microscopy (SEM and TEM).

Results : Both SEM and TEM show a number of abnormal features in the anterior lens epithelium of cataract patients with RP. The abnormalities appear mainly as holes, thinning and degradation of the epithelium, with the dimensions up to 50 um (Fig. 1). Another type of abnormalities such as cracks at the cell border (Fig. 2) were seen between adjacent LECs where lateral connections are absent, with dimensions 0.1-2 um x up to 10 um.

Conclusions : Abnormal structural features in the anterior lens epithelium were found in RP-associated cataract. This may affect the patency and transport function of the lens epithelium and cause water influx and clouding along the water clefts leading towards the posterior pole. We suggest that the lens epithelium has a role in the development of the cataract in RP patients.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

 

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×