September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Development of a custom imaging system for simultaneous monitoring of retinal and cortical physiology
Author Affiliations & Notes
  • Yusi Liu
    Vasoptic Medical Inc., Baltimore, Maryland, United States
  • Qihong Wang
    Department of Biomedical Engineering, the Johns Hopkins University, Baltimore, Maryland, United States
  • Abhishek Rege
    Vasoptic Medical Inc., Baltimore, Maryland, United States
  • Samantha Cunningham
    Vasoptic Medical Inc., Baltimore, Maryland, United States
  • Karan Raje
    Vasoptic Medical Inc., Baltimore, Maryland, United States
  • Hiren Modi
    Department of Biomedical Engineering, the Johns Hopkins University, Baltimore, Maryland, United States
  • Nitish Thakor
    Department of Biomedical Engineering, the Johns Hopkins University, Baltimore, Maryland, United States
  • Ingrid E Zimmer-Galler
    The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Peter L Gehlbach
    The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Yusi Liu, Vasoptic Medical Inc. (E), Vasoptic Medical Inc. (I); Qihong Wang, Vasoptic Medical Inc. (F); Abhishek Rege, Vasoptic Medical Inc. (E), Vasoptic Medical Inc. (I), Vasoptic Medical Inc. (P); Samantha Cunningham, Vasoptic Medical Inc. (E); Karan Raje, Vasoptic Medical Inc. (E), Vasoptic Medical Inc. (I); Hiren Modi, Vasoptic Medical Inc. (F); Nitish Thakor, Vasoptic Medical Inc. (F), Vasoptic Medical Inc. (P), Vasoptic Medical Inc. (I); Ingrid Zimmer-Galler, Vasoptic Medical Inc. (F); Peter Gehlbach, Vasoptic Medical Inc. (F)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3755. doi:
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      Yusi Liu, Qihong Wang, Abhishek Rege, Samantha Cunningham, Karan Raje, Hiren Modi, Nitish Thakor, Ingrid E Zimmer-Galler, Peter L Gehlbach; Development of a custom imaging system for simultaneous monitoring of retinal and cortical physiology. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3755.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Retinal blood flow (RBF) may offer insights into systemic health, providing physiological markers for disease and recovery. Researchers may benefit from the ability to simultaneously monitor RBF and other functional data such as systemic blood pressure (BP), electrocardiogram (ECG) and electroencephalogram (EEG) readings, and cerebral blood flow (CBF). The purpose of this study was to develop novel instrumentation for multi-parameter functional data and demonstrate feasibility by characterizing changes that accompany cardiac arrest, resuscitation and recovery.

Methods : A modular imaging platform—VasoVUE Research SystemTM—was designed to produce vessel-specific blood flow in the rat retina and cortex using laser speckle contrast imaging and high-speed photography. Image acquisition was synchronized with ECG, EEG, and BP recordings. An adult male Wistar rat (350g) was prepared by implanting EEG electrodes (right hemisphere) and exposing a thinned-skull cortical imaging window of 5mm diameter (left hemisphere). BP was measured by cannulating the left femoral artery. The rat was laid supine and the VasoVUE was oriented such that retinal and cortical vasculature was visualized clearly (Fig. 1). The rat was anesthetized with 2% isoflurane for 20 min. to establish baseline, after which a 5-min washout period (no isoflurane) was recorded. Asphyxia was induced by stopping mechanical ventilation for 6 min. Subsequently, cardiopulmonary resuscitation (CPR) was performed by ventilation, external chest compression, epinephrine and NaHCO3 until return of spontaneous circulation (ROSC, mean BP >50 mmHg). Recovery was monitored for 25 min.

Results : The VasoVUE simultaneously measured RBF and CBF with a mean coefficient of variation of 9.0% and 8.1%, respectively, and synchronously with EEG, ECG, and BP over the course of the procedure (Fig. 2). Trends in RBF and CBF were strongly correlated at baseline and during asphyxia (R2 = 0.998, p < 0.01). Trends were only weakly correlated during CPR and ROSC (R2 = 0.008, p > 0.80), requiring further investigation. A short yet temporally correlated period of elevated BP and hyperperfusion in both RBF and CBF was observed 3-4 minutes post-ROSC.

Conclusions : This study shows the feasibility of capturing RBF and CBF using the VasoVUE and utilizing these data in conjunction with multi-parameter brain and cardiovascular data for research.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

 

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