September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Cornea-mimetic lamellar microstructure and mechanical integrity of bioinspired collagen vitrigel implants
Author Affiliations & Notes
  • Shoumyo Majumdar
    Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, Maryland, United States
  • Sven Sommerfeld
    Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, Maryland, United States
  • Xiaokun Wang
    Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, Maryland, United States
  • Anirudha Singh
    Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, Maryland, United States
  • Qiongyu Guo
    Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, Maryland, United States
  • Oliver Schein
    Department of Ophthalmology, Wilmer Eye Institute, , Johns Hopkins School of Medicine, Baltimore, Maryland, United States
  • Jennifer Elisseeff
    Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, Maryland, United States
    Department of Ophthalmology, Wilmer Eye Institute, , Johns Hopkins School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Shoumyo Majumdar, None; Sven Sommerfeld, None; Xiaokun Wang, None; Anirudha Singh, None; Qiongyu Guo, None; Oliver Schein, None; Jennifer Elisseeff, None
  • Footnotes
    Support  Henry Jackson Foundation (Subaward 783536).
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 911. doi:
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    • Get Citation

      Shoumyo Majumdar, Sven Sommerfeld, Xiaokun Wang, Anirudha Singh, Qiongyu Guo, Oliver Schein, Jennifer Elisseeff; Cornea-mimetic lamellar microstructure and mechanical integrity of bioinspired collagen vitrigel implants. Invest. Ophthalmol. Vis. Sci. 2016;57(12):911.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : In recent years, researchers have moved towards biological polymers to create biocompatible corneal substitutes that can potentially integrate well with the surrounding tissue. To this end, we have developed a Type I collagen based vitrigel biomaterials that demonstrate cornea mimetic microstructure, collagen fibril alignment and lamella formation via a controlled self-assembly process using cyclodextrins as modulatory molecules. We evaluated mechanical properties of these materials and lamellar integrity via rheological analysis.

Methods : Implants were prepared using CD and Type 1 collagen solution in appropriate buffer. Samples were subjected to slow vitrification process – slow dehydration at controlled temperature and humidity for 1 week, then rehydrated in PBS. Materials were fixed & processed, and imaged with a Leo FESEM. Hydrated samples were also tested for shear strain resistance using oscillation frequency sweep on a TA Instruments Ares G2 rheometer. Further, materials were subjected to a temperature ramp at constant frequency to determine failure temperature.

Results : The FESEM images (Figure 1) demonstrated cornea mimetic lamellar formation and the alignment of collagen fibers in materials with CD. This effect is also observed in macro-scale, as the lamellae can be carefully separated. Through rheology, storage and loss modulus as a function of frequency of oscillation was obtained. It was determined that the mechanical integrity of the materials can be significantly altered using CD, and were higher than vitrigels without CD (Figure 2).

Conclusions : CD Col membranes demonstrate unique cornea-mimetic properties. Functionalization of cyclodextrins renders it possible to organize and align collagen fibrils, and to control higher order self-assembly processes. This correlates with the observed lamellar structure and fibril alignment observed in addition to the improved structural integrity observed through rheological studies.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

A) Cornea-mimetic implant fabricated with variable thickness and curvatures. B) SEM cross-sectional image on fixated sample show cornea-mimetic lamellae (scale bar = 1 μm). C) SEM angled top view image on shows aligned collagen fibril within lamellae (scale bar = 0.2 μm)

A) Cornea-mimetic implant fabricated with variable thickness and curvatures. B) SEM cross-sectional image on fixated sample show cornea-mimetic lamellae (scale bar = 1 μm). C) SEM angled top view image on shows aligned collagen fibril within lamellae (scale bar = 0.2 μm)

 

Rheological characterization of inherent membrane properties A) Storage modulus of CD-Col vitrigels B) Rheological measurements of Storage and Loss Moduli for αCD Col.

Rheological characterization of inherent membrane properties A) Storage modulus of CD-Col vitrigels B) Rheological measurements of Storage and Loss Moduli for αCD Col.

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