September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Establishment of Orthotopic Lacrimal Gland B-cell Lymphoma Model in Mice
Author Affiliations & Notes
  • Min Joung Lee
    Ophthalmology, Hallym University Sacred Heart Hospital, Anyang, Korea (the Republic of)
  • Se Yeon Park
    Laboratory of Ocular Regenerative Medicine Immunology, Biomedical Research Institute, Seoul, Korea (the Republic of)
  • Sun-Ok Yoon
    Laboratory of Ocular Regenerative Medicine Immunology, Biomedical Research Institute, Seoul, Korea (the Republic of)
  • Jung Hwa Ko
    Laboratory of Ocular Regenerative Medicine Immunology, Biomedical Research Institute, Seoul, Korea (the Republic of)
  • Hyun Ju Lee
    Laboratory of Ocular Regenerative Medicine Immunology, Biomedical Research Institute, Seoul, Korea (the Republic of)
  • Mee Kum Kim
    Laboratory of Ocular Regenerative Medicine Immunology, Biomedical Research Institute, Seoul, Korea (the Republic of)
    Ophthalmology, Seoul National University Hospital, Seoul, Korea (the Republic of)
  • Won Ryang Wee
    Laboratory of Ocular Regenerative Medicine Immunology, Biomedical Research Institute, Seoul, Korea (the Republic of)
    Ophthalmology, Seoul National University Hospital, Seoul, Korea (the Republic of)
  • Sang In Khwarg
    Ophthalmology, Seoul National University Hospital, Seoul, Korea (the Republic of)
  • Joo Youn Oh
    Laboratory of Ocular Regenerative Medicine Immunology, Biomedical Research Institute, Seoul, Korea (the Republic of)
    Ophthalmology, Seoul National University Hospital, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Min Joung Lee, None; Se Yeon Park, None; Sun-Ok Yoon, None; Jung Hwa Ko, None; Hyun Ju Lee, None; Mee Kum Kim, None; Won Ryang Wee, None; Sang In Khwarg, None; Joo Youn Oh, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4103. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Min Joung Lee, Se Yeon Park, Sun-Ok Yoon, Jung Hwa Ko, Hyun Ju Lee, Mee Kum Kim, Won Ryang Wee, Sang In Khwarg, Joo Youn Oh; Establishment of Orthotopic Lacrimal Gland B-cell Lymphoma Model in Mice. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4103.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To develop and characterize a lacrimal gland B-cell lymphoma model in mice.

Methods : Either 1x105, or 1x106 syngeneic lymphomatous cell line (A20 cell line) suspended in 20ul phosphate buffer solution were injected into intraorbital and extraorbital lacrimal glands. Lacrimal glands were dissected and the volume was measured at 1, 2, 3, and 4 weeks after injection. Infiltration of tumor cells and damage to the lacrimal glands were evaluated by histopathology. Also, the cellular components of tumor microenvironment were studied by immunofluorescence staining.

Results : The volume of intraorbital lacrimal glands was significantly larger in 1x106 A20 group at 1 week after injection (p=0.0117 vs no injection group, p=0.0081 vs 1x105 A20 group). The volume of extraorbital lacrimal was also significantly increased in 1x106 A20 group at 1 week (p=0.009 vs no injection group, p=0.0040 vs 1x105 A20 group) and 2 week (p=0.0044 vs no injection group, p=0.0283 vs 1x105 A20 group) after injection. The volume of lacrimal glands was not significantly different among three groups 3 weeks and 4 weeks after injection. Tumor cells intensively invaded and diffusely observed in whole intraorbital and extraorbital lacrimal gland specimens obtained at 1 and 2 weeks after injection. Lacrimal glands at 3 weeks and 4 weeks showed only focal tumor cell infiltration. CD19+ B cells were abundantly infiltrated in lacrimal glands at 1 week and 2 weeks. CD3+ T cells and CD11b+ myeloid-derived suppressor cells were accompanied with tumor.

Conclusions : We established lacrimal gland B-cell lymphoma model in mice by orthotopic injection of A20 cells. The tumorigenesis effect was greater in high dose injection group, and the time course peaked 1 week after injection in intraorbital glands, and 2 weeks in extraorbital glands.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

The volume of intraorbital lacrimal glands was significantly higher in 1x106 A20 injection group at 1week after injection. In case of extraorbital lacrimal glands, the volume was greater in 1x106 A20 grou at 1 week and 2 weeks after injection.

The volume of intraorbital lacrimal glands was significantly higher in 1x106 A20 injection group at 1week after injection. In case of extraorbital lacrimal glands, the volume was greater in 1x106 A20 grou at 1 week and 2 weeks after injection.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×