September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Corneal Wound Healing after Small Incision Lenticule Extraction (SMILE)
Author Affiliations & Notes
  • Yuan Sun
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Ting Zhang
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Manli Liu
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Yugui Zhou
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Shengbei Weng
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Xiaonan Yang
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Chi-ho To
    School of Optometry, The Hong Kong Polytechnic University, Hong Kong, Hong Kong
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Quan Liu
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
    School of Optometry, The Hong Kong Polytechnic University, Hong Kong, Hong Kong
  • Footnotes
    Commercial Relationships   Yuan Sun, None; Ting Zhang, None; Manli Liu, None; Yugui Zhou, None; Shengbei Weng, None; Xiaonan Yang, None; Chi-ho To, None; Quan Liu, None
  • Footnotes
    Support  Supported by grants from the Natural Science Foundation of China (81261130021), the Science and Technology Program of Guangzhou (2013B 021800126) and the Natural Science Foundation of Guangdong (2015A 030310098).
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4879. doi:
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      Yuan Sun, Ting Zhang, Manli Liu, Yugui Zhou, Shengbei Weng, Xiaonan Yang, Chi-ho To, Quan Liu; Corneal Wound Healing after Small Incision Lenticule Extraction (SMILE). Invest. Ophthalmol. Vis. Sci. 2016;57(12):4879.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the wound healing response after SMILE procedure in rabbit model.

Methods : SMILE was performed in both eyes of 12 New Zealand White rabbits. The cap diameter and thickness were 7.5 mm and 100 μm, respectively. The refractive spherical correction was set at -6.00 D. These animals were divided into 6 groups and the follow-up examinations were performed 1 hour, 4 hour and 1 day, 3 days, 7 days and 28 days after surgery. Corneas were evaluated using slit lamp and in vivo confocal microscopy. The enucleated eyes were used for light microscopy, terminal deoxyribonucleotidyl transferasemediated dUTP-digoxigenin nick-end labelling (TUNEL) assay and immunofluorescence staining (Fibronectin, Tenascin C, CD11b, alpha-SMA).

Results : The central corneas did not show any noticeable opacity at any time point of follow-up. In the contrast, there were obvious scar tissues at the incision position. Scattering hyper reflective spots were observed through in vivo confocal microscopy at 4 hours postoperatively. Large sums of TUNEL-positive keratocytes were detected near the laser ablation zone at 1 hour after surgery and decreased afterwards. Inflammation cells were found to enter from the incision into the central cornea. The inflammatory reaction was observed from 1 hour after surgery. The deposition of extracellular matrix (ECM; Fibronectin & Tenascin) was detected at 1 day postoperatively. The distribution pattern of ECM between the central cornea and the incision was different. Alpha-SMA-positive fibroblasts were detected at the incision position, but it was not observed at central cornea.

Conclusions : It is necessary to implement anti-inflammation intervention at early stage after surgery. The non-scar wound healing response was resulted in the different reaction patterns between the central cornea and the incision.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

 

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