September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Regionally Discrete Aqueous Humor Outflow Quantification using Fluorescein Canalograms
Author Affiliations & Notes
  • Eric N Brown
    Department of Ophthalmology, Vanderbilt University, Nashville, Tennessee, United States
  • Ralitsa Loewen
    Department of Ophthalmology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
    The Louis J Fox Center for Vision Restoration, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Pritha Sengupta
    Department of Ophthalmology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
    The Louis J Fox Center for Vision Restoration, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Joel S Schuman
    Department of Ophthalmology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
    The Louis J Fox Center for Vision Restoration, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Ian A Sigal
    Department of Ophthalmology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
    The Louis J Fox Center for Vision Restoration, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Nils A Loewen
    Department of Ophthalmology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
    The Louis J Fox Center for Vision Restoration, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Footnotes
    Commercial Relationships   Eric Brown, None; Ralitsa Loewen, None; Pritha Sengupta, None; Joel Schuman, None; Ian Sigal, None; Nils Loewen, None
  • Footnotes
    Support  National Eye Institute KO8-EY022737; Research to Prevent Blindness; National Eye Institute 5R01EY013178-16; National Eye Institute 2T32EY017271-06A1 (IVS Training Program); National Eye Institute 5R01EY023966-02
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3541. doi:
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    • Get Citation

      Eric N Brown, Ralitsa Loewen, Pritha Sengupta, Joel S Schuman, Ian A Sigal, Nils A Loewen; Regionally Discrete Aqueous Humor Outflow Quantification using Fluorescein Canalograms. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3541.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To quantify conventional outflow directly in its anatomic location in a common anterior segment culture model.

Methods : We obtained fluorescein canalograms in six porcine whole eyes (WhEs) and six porcine anterior segment cultures (ACs). Eyes were perfused with a constant pressure of 15 mmHg using media containing 0.017 mg/ml fluorescein. Flow patterns were visualized using a stereo dissecting microscope equipped for fluorescent imaging as previously described. Canalograms were analyzed for filling time per quadrant using a custom designed R software package and a generalized additive model. Changes in outflow was quantified and compared over time in anterior segment cultures. The code and examples of its use will be publically available at GitHub.com.

Results : In WhEs, quantifiably faster flow was seen nasally compared to other regions. ACs had reduced numbers of functional collectors on day 1 without exhibiting a preferential flow pattern. Statistical analysis revealed correlations and increase in nasal filling in ACs on day 3. Automated imaging analysis algorithm classified areas of perilimbal outflow based on the total amount of flow and the rate of flow. Filling rates uncovered convergent perilimbal flow.

Conclusions : An algorithm was developed that analyzes regional and circumferential outflow patterns. Altered flow was detected in a common anterior segment culture model.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

Quantification of conventional aqueous humor outflow.

Quantification of conventional aqueous humor outflow.

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