September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
En Face Doppler OCT Measurement of Total Retinal Blood Flow in Eyes with Diabetic Retinopathy and Diabetic Macular Edema
Author Affiliations & Notes
  • ByungKun Lee
    Department of Electrical Engineering and Computer Science / Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
  • Eduardo Amorim Novais
    New England Eye Center, Department of Ophthalmology, Tufts University School of Medicine, Boston, Massachusetts, United States
  • Nadia K Waheed
    New England Eye Center, Department of Ophthalmology, Tufts University School of Medicine, Boston, Massachusetts, United States
  • Mehreen Adhi
    New England Eye Center, Department of Ophthalmology, Tufts University School of Medicine, Boston, Massachusetts, United States
  • Talisa E de Carlo
    New England Eye Center, Department of Ophthalmology, Tufts University School of Medicine, Boston, Massachusetts, United States
  • WooJhon Choi
    Department of Electrical Engineering and Computer Science / Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
  • Eric M Moult
    Department of Electrical Engineering and Computer Science / Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
  • Jay S Duker
    New England Eye Center, Department of Ophthalmology, Tufts University School of Medicine, Boston, Massachusetts, United States
  • James G Fujimoto
    Department of Electrical Engineering and Computer Science / Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
  • Footnotes
    Commercial Relationships   ByungKun Lee, None; Eduardo Novais, None; Nadia Waheed, Carl Zeiss Meditec, Inc. (F), Iconic Therapeutics, Inc. (C), ThromboGenics NV (C); Mehreen Adhi, None; Talisa de Carlo, None; WooJhon Choi, None; Eric Moult, None; Jay Duker, Carl Zeiss Meditec, Inc. (F), Carl Zeiss Meditec, Inc. (C), Optovue, Inc. (F), Optovue, Inc. (C); James Fujimoto, Carl Zeiss Meditec, Inc. (P), Optovue, Inc. (I), Optovue, Inc. (P)
  • Footnotes
    Support  NIH R01-EY011289-29A, R44-EY022864, R01-CA075289-16; AFOSR FA9550-15-1-0473, FA9550-12-1-0499
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5921. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      ByungKun Lee, Eduardo Amorim Novais, Nadia K Waheed, Mehreen Adhi, Talisa E de Carlo, WooJhon Choi, Eric M Moult, Jay S Duker, James G Fujimoto; En Face Doppler OCT Measurement of Total Retinal Blood Flow in Eyes with Diabetic Retinopathy and Diabetic Macular Edema. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5921.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Cross-sectional, observational study to investigate total retinal blood flow (TRBF) using en face Doppler optical coherence tomography (OCT) in eyes with diabetic macular edema (DME), non-proliferative diabetic retinopathy (NPDR), and proliferative diabetic retinopathy (PDR).

Methods : TRBF was measured in 33 eyes of 18 patients with diabetic retinopathy (DR) (8 females, age 64.5±12.9 years), and 16 eyes of 12 normal control subjects (8 females, age 59.3±10.4 years), using a 1050-nm swept-source OCT prototype operating at 400-kHz axial scan rate. Volumetric data comprising 600×80 axial scans over a 1.5mm×2mm area at the optic disc was repeatedly acquired 24 times in 3.4 seconds. TRBF was automatically calculated and averaged over the cardiac cycle (Fig. 1). The patients were stratified by disease severity into mild NPDR, moderate NPDR, and severe NPDR or PDR as well as by the presence or absence of DME. Among the 13 eyes with DME, 3 were not treated prior to imaging, while other 3 received anti-VEGF only, 4 received focal laser only, and 3 received both anti-VEGF and laser.

Results : Mean TRBF in the normal group, the DME group, and the DR without DME group was 44.4±8.3µL/min, 29.3±9.4µL/min, and 51.7±16.4µL/min, respectively (Fig. 2A). TRBF was significantly lower in the eyes with DME compared to both the normal eyes (p≤0.001, Welch’s t-test) and the DR eyes without DME (p≤0.002). Within the 20 DR eyes without DME, TRBF in the mild NPDR, moderate NPDR, and severe NPDR/PDR groups was 45.4±9.18µL/min, 49.5±19.1µL/min, and 55.4±11.7µL/min, respectively. Within the 13 eyes with DME, TRBF in the mild NPDR, moderate NPDR, and severe NPDR/PDR groups was 32.3µL/min, 30.7±10.2µL/min, and 25.2±6.2µL/min, respectively (Fig. 2B).

Conclusions : TRBF in DR patients was measured by high-speed en face Doppler OCT. A decrease in TRBF was observed in eyes with DME compared to DR eyes without DME and normal eyes. Further investigation is merited as our observations suggest the complex nature of TRBF changes associated with DR progression.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

Fig. 1. TRBF measurement in a patient with unilateral DME. (A, D) En face Doppler OCT scan area marked on color fundus photographs. (B, C, E, F) Flow velocity en face profiles at systole and diastole. (G) Pulsatile TRBF in the two eyes. Scale bars 500µm.

Fig. 1. TRBF measurement in a patient with unilateral DME. (A, D) En face Doppler OCT scan area marked on color fundus photographs. (B, C, E, F) Flow velocity en face profiles at systole and diastole. (G) Pulsatile TRBF in the two eyes. Scale bars 500µm.

 

Fig. 2. Box plots showing the median and quartiles of mean TRBF in each group.

Fig. 2. Box plots showing the median and quartiles of mean TRBF in each group.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×