September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Multifunctional OCT Imaging Facility for Rodent Eye Imaging
Author Affiliations & Notes
  • Jens Horstmann
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
    Cluster of Excellence: Cellular Stress Responses in Aging-associated Diseases (CECAD), Cologne, Germany
  • Uta Gehlsen
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
    Cluster of Excellence: Cellular Stress Responses in Aging-associated Diseases (CECAD), Cologne, Germany
  • Sebastian E Siebelmann
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
    Cluster of Excellence: Cellular Stress Responses in Aging-associated Diseases (CECAD), Cologne, Germany
  • Deniz Hos
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
  • Felix Bock
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
  • Rafael S Grajewski
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
  • Sascha Fauser
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
  • Thomas Langmann
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
  • Gereon Huttmann
    Institute of Biomedical Optics, University of Luebeck, Luebeck, Germany
  • Claus Cursiefen
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
  • Philipp Steven
    Department of Ophthalmology, University Hospital Cologne, Cologne, Germany
    Cluster of Excellence: Cellular Stress Responses in Aging-associated Diseases (CECAD), Cologne, Germany
  • Footnotes
    Commercial Relationships   Jens Horstmann, None; Uta Gehlsen, None; Sebastian Siebelmann, None; Deniz Hos, None; Felix Bock, None; Rafael Grajewski, None; Sascha Fauser, None; Thomas Langmann, None; Gereon Huttmann, None; Claus Cursiefen, None; Philipp Steven, None
  • Footnotes
    Support  DFG Grant STE1928/4-1, Helmut und Ruth Lingen-Stiftung
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2193. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Jens Horstmann, Uta Gehlsen, Sebastian E Siebelmann, Deniz Hos, Felix Bock, Rafael S Grajewski, Sascha Fauser, Thomas Langmann, Gereon Huttmann, Claus Cursiefen, Philipp Steven; Multifunctional OCT Imaging Facility for Rodent Eye Imaging. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2193.

      Download citation file:


      © 2017 Association for Research in Vision and Ophthalmology.

      ×
  • Supplements
Abstract

Purpose : Limitations exist in commercial devices for experimental rodent eye imaging as software algorithms and hardware components are not easy to modify and to transfer into the clinic. The aim of this project was to design and establish a highly flexible multimodal Optical Coherence Tomography (OCT) facility for the development of novel methods and algorithms for in vivo OCT 2D and 3D imaging, OCT-Angiography (OCA) and ocular surface inflammation quantification using different rodent models.

Methods : The system was designed by the manufacturer of a commercially available OCT device (iOCT, OptoMedical Technologies GmbH, Luebeck, Germany) for high quality OCT and fluorescence imaging of the anterior and posterior segment. The optical coherence and fluorescence angiography mode allows co-registered imaging of vessels. In addition, the bright field mode enables clinical evaluation of the specimen.

Results : In vivo images of different mouse models for e.g. corneal and retinal neovascularization, uveitis and ocular allergy demonstrate the functionality of the device. Corneal and conjunctival blood vessels can be recorded by OCA. The results are co-registered by fluorescence angiography. To allow for exact measurements of e.g. corneal thickness or volume, the images are processed using an automatic geometric correction algorithm.

Conclusions : In contrast to commercially available systems for small animal eye imaging, the realized system allows modification and in-depth analysis of the data. This enables evaluation and development of advanced OCT imaging modalities such as Doppler, Speckle variance or Polarization sensitive OCT and early in vivo studies. Due to the technical similarity to a certified and clinically established device, fast translation of new approaches into the clinic is promoted.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

a) Experimental OCT facility, b) OCA of vascularized murine cornea, c) Cross-sectional OCT image of murine anterior chamber

a) Experimental OCT facility, b) OCA of vascularized murine cornea, c) Cross-sectional OCT image of murine anterior chamber

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×