September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Preclinical Single photon emission computered tomography/computered tomography(SPECT/CT) imaging of choroidal neovascularization in macular degeneration mice using integrin-binding 99mTc-IDA-D-[c(RGDfK)]2
Author Affiliations & Notes
  • Hye kyoung Hong
    Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Korea (the Republic of)
  • Seong Joon Ahn
    Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Korea (the Republic of)
  • Se Joon Woo
    Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Korea (the Republic of)
  • Kyu Hyung Park
    Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Korea (the Republic of)
  • ji hyun park
    Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Korea (the Republic of)
  • Jae Ho Jeong
    Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Korea (the Republic of)
  • Byung Chul Lee
    Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Hye kyoung Hong, None; Seong Joon Ahn, None; Se Joon Woo, None; Kyu Hyung Park, None; ji hyun park, None; Jae Ho Jeong, None; Byung Chul Lee, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2202. doi:
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      Hye kyoung Hong, Seong Joon Ahn, Se Joon Woo, Kyu Hyung Park, ji hyun park, Jae Ho Jeong, Byung Chul Lee; Preclinical Single photon emission computered tomography/computered tomography(SPECT/CT) imaging of choroidal neovascularization in macular degeneration mice using integrin-binding 99mTc-IDA-D-[c(RGDfK)]2. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2202.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Purpose : Integrin alpha Vbeta3 is preferentially increased on neovascular endothelial cells and is involved in ocular angiogenesis. We explored the use of integrin alphaVbeta3-targeted RGD-peptide for in vivo imaging of choroidal neovascularization and evaluated the serial angiogenic activity of 99mTc-IDA-D-[c(RGDfK)]2 for detecting the lesion of laser-irradiated choroidal tissues.

Methods : Male C57Bl/6 mice were treated with retinal argon laser photocoagulation to induce CNV. Mice were intravenously injected with 99mTc-IDA-D-[c(RGDfK)]2, synthesized by incorporating [99mTc(CO)3]+ into the precursor (IDA-D-[c(RGDfK)]2). Images of 99mTc-IDA-D-[c(RGDfK)]2 was obtained before laser induction (0 day) and 1, 3, 7, and 14 days after the induction. Regional alterations in the eye of CNV models were measured from the in vivo uptake of 99mTc-IDA-D-[c(RGDfK)]2 images created by region of intest (ROI) using a small animal single-photon emission computed tomography (SPECT). Integrin mRNA expression in the retinal andchorodial tissue was measured by reverse transcriptase-polymerase chain reaction (RT-PCR). Histopatholgy and fluorescent staining assay were performed on in vivo imgaged retina/choroidal tissues with an intense 99mTc-IDA-D-[c(RGDfK)]2 signal using anti-ICAM2, Lectin-BS1-TRITC, anti-alphaVbeta3 integrin, and FITC-D-[c(RGDfK)]2

Results : The radio-uptake count in eyes with induced CNV was significantly higher than the fellow eye from Day 1 to Day 14 (all p <0.05) and peaked at Day 3. Histopathological characterization revealed that a prominent SPECT signal of 99mTc-IDA-D-[c(RGDfK)]2 corresponded to the presence of choroidal neovascularization with integrin alphaV beta3 and intercellular adhesion molecule 2 expression. Integrin alphaVbeta3 mRNA expression was remarkably increased at Day 1.

Conclusions : Our results demonstrated that 99mTc-IDA-D-[c(RGDfK)]2 SPECT is a sensitive and integrin- targeted probe for noninvasively detecting the CNV event.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

 

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