September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Dynamic Interaction of the Ciliary Muscle and Crystalline Lens during Accommodation evaluated with SD-OCT
Author Affiliations & Notes
  • Marco Ruggeri
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Carolina De Freitas
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Siobhan Williams
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Biomedical Optics and Laser Laboratory, Department of Biomedical Engineering, University of Miami, College of Engineering, Coral Gables, Florida, United States
  • Victor M Hernandez
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Biomedical Optics and Laser Laboratory, Department of Biomedical Engineering, University of Miami, College of Engineering, Coral Gables, Florida, United States
  • Florence Cabot
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Anne Bates Leach Eye Hospital, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Karam Alawa
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Yu-Cherng Chang
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Biomedical Optics and Laser Laboratory, Department of Biomedical Engineering, University of Miami, College of Engineering, Coral Gables, Florida, United States
  • Giovanni Gregori
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Sonia H Yoo
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Anne Bates Leach Eye Hospital, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Jean-Marie A Parel
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Brien Holden Vision Institute and Vision Cooperative Research Centre, Sydney, New South Wales, Australia
  • Fabrice Manns
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Biomedical Optics and Laser Laboratory, Department of Biomedical Engineering, University of Miami, College of Engineering, Coral Gables, Florida, United States
  • Footnotes
    Commercial Relationships   Marco Ruggeri, US8425037 B2 (P); Carolina De Freitas, None; Siobhan Williams, None; Victor Hernandez, None; Florence Cabot, None; Karam Alawa, None; Yu-Cherng Chang, None; Giovanni Gregori, None; Sonia Yoo, None; Jean-Marie Parel, US8425037 B2 (P); Fabrice Manns, US8425037 B2 (P)
  • Footnotes
    Support  National Eye Institute Grants 2R01EY14225, P30EY14801 (Center Grant); Florida Lions Eye Bank; Drs KR Olsen and ME Hildebrandt; Drs. Raksha Urs and Aaron Furtado; the Henri and Flore Lesieur Foundation (JMP); an unrestricted grant from Research to Prevent Blindness; and the Australian Federal Government Cooperative Research Centre Scheme through the Vision Cooperative Research Centre.
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3960. doi:
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    • Get Citation

      Marco Ruggeri, Carolina De Freitas, Siobhan Williams, Victor M Hernandez, Florence Cabot, Karam Alawa, Yu-Cherng Chang, Giovanni Gregori, Sonia H Yoo, Jean-Marie A Parel, Fabrice Manns; Dynamic Interaction of the Ciliary Muscle and Crystalline Lens during Accommodation evaluated with SD-OCT. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3960.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the dynamic interaction between the human ciliary muscle and crystalline lens during accommodation using SD-OCT.

Methods : We combined and synchronized two SD-OCT systems individually operating at 840nm and 1325nm and an accommodation unit that provides monocular accommodation step stimuli. Imaging of the anterior segment including cornea, anterior chamber and crystalline lens was performed with the SD-OCT system at 840nm. The SD-OCT system at 1325nm was used for transscleral imaging of the ciliary muscle. The two SD-OCT systems enable simultaneous and synchronized acquisition of two OCT image sequences of, respectively, the ciliary muscle and the crystalline lens at an imaging speed of 13 frames per second. The response to an accommodative step stimulus varying between 0D and 2D was imaged in the left eye of a 22 and a 45 year-old subjects. Each OCT image sequence consisted of 80 frames. Segmentation and distortion correction of the ocular boundaries was performed on each OCT frame to extract the time dependent crystalline lens thickness (LT) and ciliary muscle maximum thickness (CMT).

Results : The system displays the synchronous response of the ciliary muscle and crystalline lens in real-time. Graphs plotting the dynamic changes of LT in function of CMT (ΔLT vs. ΔCMT) during accommodation were obtained. At the end of the accommodative process, the mean ΔCMT and ΔLT were, respectively, 0.045mm and 0.125mm for the 22 year-old subject and 0.107mm and 0.090mm for the 45 year-old subject. We calculated the ratio ΔLT/ΔCMT during dynamic accommodation as the slope of the linear regression of the data points (ΔCMT, ΔLT) during the rising phase of the lens thickness. ΔLT/ΔCMT was 2.268 in the 22 year-old subject and 0.746 in the 45 year-old subject. The change in ciliary muscle thickness required to elicit a ΔLT of 5% during the initial stage of the rising phase (ΔCMTΔLT5%) was 0.005mm and 0.034mm for the 22 and the 45 year-old subject, respectively.

Conclusions : This study demonstrates the feasibility of imaging and quantifying the interaction between the ciliary muscle and crystalline lens during accommodation in subjects with different ages. The preliminary results suggest that a larger effort of the ciliary muscle is required to elicit the same change in lens thickness in an older lens.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

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