September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
En-face imaging as a method for monitoring changes in the inner segment (IS)/outer segment (OS) band in retinitis pigmentosa
Author Affiliations & Notes
  • Jason Nunez
    Psychology , Columbia University, New York City, New York, United States
  • Donald Charles Hood
    Psychology , Columbia University, New York City, New York, United States
    Ophthalmology, Columbia University, New York , New York, United States
  • Daiyan Xin
    Psychology , Columbia University, New York City, New York, United States
  • Stephen H Tsang
    Ophthalmology, Columbia University, New York , New York, United States
    Pathology and Cell Biology, Columbia University, New York, New York, United States
  • Juan Reynaud
    Legacy Devers Eye Institute, Portland, Oregon, United States
  • David G Birch
    Retina Foundation of the Southwest, Dallas, Texas, United States
  • Brad Fortune
    Legacy Devers Eye Institute, Portland, Oregon, United States
  • Vivienne C. Greenstein
    Ophthalmology, Columbia University, New York , New York, United States
  • Footnotes
    Commercial Relationships   Jason Nunez, None; Donald Hood, Heidelberg Engineering (F), Topcon, Inc. (F), Topcon, Inc. (C), Zeiss (C); Daiyan Xin, None; Stephen Tsang, None; Juan Reynaud, None; David Birch, None; Brad Fortune, None; Vivienne Greenstein, None
  • Footnotes
    Support  R01 EY009076
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 142. doi:
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    • Get Citation

      Jason Nunez, Donald Charles Hood, Daiyan Xin, Stephen H Tsang, Juan Reynaud, David G Birch, Brad Fortune, Vivienne C. Greenstein; En-face imaging as a method for monitoring changes in the inner segment (IS)/outer segment (OS) band in retinitis pigmentosa. Invest. Ophthalmol. Vis. Sci. 2016;57(12):142.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To compare en-face imaging of the area of the intact IS/OS band (aka EZ band) derived from wide-field swept-source optical coherence tomography (ssOCT) volume scans to current measurements of IS/OS band width on OCT averaged B-scans, and measurements of the borders of rings/arcs seen on short-wavelength fundus autofluorescent (SW-FAF) images in patients with retinitis pigmentosa (RP).

Methods : Wide-field, ssOCT cube scans (9 x12 mm, 256 B-scans, 512 A-scans, DRI-OCT, Topcon, Inc) were obtained from 7 eyes of 6 patients with RP (autosomal recessive: 2, autosomal dominant: 2, Usher syndrome type II: 2) aged 19 to 60 years with BCVAs ranging from 20/20 to 20/25. OCT macular volume and averaged B-scans, and SW-FAF images, were also obtained using Spectralis HRA+OCT (Heidelberg Eng). Following manual correction of the automated Topcon segmentation of the IS/OS band and OS/retinal pigment epithelium (OS/RPE) layers, the average reflective intensity of en-face slabs of varying thickness were generated with these borders as references using special purpose software (ATL 3D-Suite).[1,2] For each eye, the lateral extent of the intact IS/OS area was compared to the width of the band measured on OCT B-scans and to the borders of the hyperFAF ring/arc on the SW-FAF image following registration of the images.

Results : For the en-face images (Fig. 1B), slabs (Fig. 1A), with the IS/OS band as reference, optimized visualization of the boundary of the intact IS/OS area for 6 eyes (Fig. 1B); for one eye, the reference was the OS/RPE. For all 7 eyes, there was good agreement between the lateral extent of the IS/OS area and width of the band measured on the averaged B-scans (Fig.1B, C, solid vertical lines), as well as with the locations of the terminations of the band on individual ssOCT B-scans. In addition, all 7 eyes had a ring/arc of hyperFAF (Fig. 1D). The inner border of the hyperFAF area (Fig. 1D) corresponded to the en-face boundary of the IS/OS area (Fig. 1B).

Conclusions : En-face imaging has potential clinical value for visualizing and tracking changes in the integrity of the IS/OS band in patients with RP. 1. Fortune et al. IOVS 2014; 2. Hood, Fortune et al. IOVS 2015.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

Fig. 1. A. B-scan from cube. B. En-face slab. C. Averaged B-scan. D. SW-FAF.

Fig. 1. A. B-scan from cube. B. En-face slab. C. Averaged B-scan. D. SW-FAF.

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