September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Adeno-associated viral vector labeling of retinal ganglion cells for in vivo imaging
Author Affiliations & Notes
  • Corey A Smith
    Physiology and Biophysics/Ophthalmology and Visual Sciences, Dalhousie University, Halifax, Nova Scotia, Canada
  • Michele L Hooper
    Nova Scotia Health Authority, Halifax, Nova Scotia, Canada
  • Spring R Farrell
    Nova Scotia Health Authority, Halifax, Nova Scotia, Canada
  • Balwantray C Chauhan
    Physiology and Biophysics/Ophthalmology and Visual Sciences, Dalhousie University, Halifax, Nova Scotia, Canada
    Nova Scotia Health Authority, Halifax, Nova Scotia, Canada
  • Footnotes
    Commercial Relationships   Corey Smith, None; Michele Hooper, None; Spring Farrell, None; Balwantray Chauhan, Heidelberg Engineering (F)
  • Footnotes
    Support  Atlantic Canada Opportunities Agency, Atlantic Innovation Fund 197809
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 1712. doi:
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    • Get Citation

      Corey A Smith, Michele L Hooper, Spring R Farrell, Balwantray C Chauhan; Adeno-associated viral vector labeling of retinal ganglion cells for in vivo imaging. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1712.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Purpose : The ability to detect and measure early retinal ganglion cell (RGC) loss is important for the diagnosis and monitoring of diseases such as glaucoma. We tested the hypothesis that adeno-associated viral (AAV) vectors would facilitate green fluorescent protein (GFP) labeling of RGCs in mice for in vivo non-invasive and real-time imaging as a means of RGC quantification.

Methods : 1.5 μl of vector with a ubiquitous (AAV2-CAG-GFP) or RGC selective (AAV2-DCX-GFP) promoter was injected into the vitreous of the left eye of C57BL/6 mice (n = 6). The retina was imaged non-invasively by fluorescence imaging (488 nm excitation) with a modified Spectralis (Heidelberg Engineering) device weekly post-injection. All procedures were performed in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Visual Research. Immunohistochemistry was performed in retinal flat-mount and section preparations for RGC (RNA binding protein with multiple splicing, RBPMS) and amacrine (choline acetyltransferase, ChAT) cell labelling, respectively.

Results : In vivo imaging detected fluorescently labeled cells 1-week post-injection of either vector. Labeling was visible in vivo continuously for at least 5 weeks. Expression profiles demonstrated increased transduction occurred up until approximately 4 weeks post-injection. The in vivo mean (standard error, SE) central retina cell density for the AAV2-CAG-GFP vector was 97 (25) cells/mm2 at week-1 and 638 (90) cells/mm2 at week-4. For the AAV2-DCX-GFP vector, cell density was 29 (20) cells/mm2 at week-1 and 288 (54) cells/mm2 at week-4. Immunohistochemistry demonstrated that 5-weeks post-injection, the mean (SE) GFP+ cells that were RBPMS+ was significantly higher (p < 0.05) for the AAV2-DCX-GFP vector, 89 (2)%, compared to the AAV2-CAG-GFP vector, 76 (2)%.

Conclusions : AAV mediated GFP expression labeled RGCs with strong and long-lasting fluorescence when administered by intravitreal injection. Furthermore, AAV specificity to RGCs can be improved with tissue specific promoters such as DCX. This labeling and longitudinal imaging technique can be used to confirm the presence of healthy RGCs and detect changes in the number of RGCs over time.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

In vivo images of GFP labeled retinal neurons in mouse post-intravitreal injection of a ubiquitous (AAV2-CAG-GFP) or RGC selective (AAV2-DCX-GFP) vector.

In vivo images of GFP labeled retinal neurons in mouse post-intravitreal injection of a ubiquitous (AAV2-CAG-GFP) or RGC selective (AAV2-DCX-GFP) vector.

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