September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Comparing three different modes of electroretinography in experimental glaucoma: diagnostic performance and correlation to structure
Author Affiliations & Notes
  • Laura J Wilsey
    Dept of Eye and Vision Science, Legacy Research Institute, Legacy Health, Portland, Oregon, United States
  • Sowjanya Gowrisankaran
    Department of Ophthalmology, Boston Children’s Hospital, Boston, Massachusetts, United States
  • Grant Cull
    Dept of Eye and Vision Science, Legacy Research Institute, Legacy Health, Portland, Oregon, United States
  • Christy A Hardin
    Dept of Eye and Vision Science, Legacy Research Institute, Legacy Health, Portland, Oregon, United States
  • Claude F Burgoyne
    Dept of Eye and Vision Science, Legacy Research Institute, Legacy Health, Portland, Oregon, United States
  • Brad Fortune
    Dept of Eye and Vision Science, Legacy Research Institute, Legacy Health, Portland, Oregon, United States
  • Footnotes
    Commercial Relationships   Laura Wilsey, None; Sowjanya Gowrisankaran, None; Grant Cull, None; Christy Hardin, None; Claude Burgoyne, Heidelberg Engineering, GmbH (F), Heidelberg Engineering, GmbH (C), Heidelberg Engineering, GmbH (R); Brad Fortune, None
  • Footnotes
    Support  NIH R01-EY019327 (BF), R01-EY011610 (CFB); Legacy Good Samaritan Foundation
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 386. doi:
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    • Get Citation

      Laura J Wilsey, Sowjanya Gowrisankaran, Grant Cull, Christy A Hardin, Claude F Burgoyne, Brad Fortune; Comparing three different modes of electroretinography in experimental glaucoma: diagnostic performance and correlation to structure. Invest. Ophthalmol. Vis. Sci. 2016;57(12):386.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To compare diagnostic performance and structure-function correlations of multifocal electroretinogram (mfERG), full-field flash ERG (ff-ERG) photopic negative response (PhNR) and transient pattern-reversal ERG (PERG) in a non-human primate (NHP) model of experimental glaucoma (EG).

Methods : At baseline and after induction of chronic unilateral IOP elevation, 43 NHP had weekly recordings of retinal nerve fiber layer thickness (RNFLT) by SDOCT (Spectralis) and retinal function by mfERG (7F slow-sequence stimulus, VERIS), ff-ERG (red 0.42 log cd-sec/m2 flashes on blue 30 scotopic cd/m2 background, LKC UTAS-E3000), and PERG (0.8° checks, 99% contrast, 100 cd/m2 mean, 5 reversals/sec, VERIS). All NHP were followed at least until HRT-confirmed optic nerve head posterior deformation, most to later stages. mfERG responses were filtered into low- and high-frequency components (LFC, HFC, >75 Hz). Peak-to-trough amplitudes of LFC features (N1, P1, N2) and HFC RMS amplitudes were measured and ratios calculated for HFC:P1 and N2:P1. ff-ERG parameters included A-wave (at 10 ms), B-wave (trough-to-peak) and PhNR (at 70 ms) amplitudes as well as PhNR:B-wave ratio. PERG parameters included P50 and N95 amplitudes as well as N95:P50 ratio and N95 slope. Diagnostic performance of retinal function parameters were compared using the area under the receiver operating characteristic curve (AROC) to discriminate between EG and control eyes. Correlations to RNFLT were compared using Steiger’s test.

Results : Study duration was 15 ± 8 mos. At final follow-up, structural damage in EG eyes measured by RNFLT ranged from 9% above BL to 58% below BL; 29/43 EG eyes (67%) and 0/43 of the fellow control eyes exhibited significant (>7%) loss of RNFLT from BL. Using raw parameter values, the largest AROC findings for mfERG were: HFC (0.81) and HFC:P1 (0.88); for ff-ERG: PhNR (0.73) and PhNR:B-wave (0.81) and for PERG: N95 (0.60); see Table 1A and Fig1A. AROC increased when data were expressed as % change from BL (Table 1B and Fig1B) but the pattern of results persisted: mfERG HFC was best, followed by PhNR and PERG. The correlation to RNFLT was signficantly higher for mfERG HFC (R=0.65) than for PhNR (0.42, p=0.003) or PERG N95 (0.35, p=0.001).

Conclusions : Diagnostic performance and structure-function correlations were strongest for mfERG HFC as compared with ff-ERG PhNR or PERG in NHP EG.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

 

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