September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Retinal vasculature in mice: A comparison of optical coherence tomography angiography and confocal scanning laser microscopy
Author Affiliations & Notes
  • Helena Zimmermann
    Ophthalmology, Inselspital Bern, Bern, Switzerland
  • Despina Kokona
    Ophthalmology, Inselspital Bern, Bern, Switzerland
  • Sebastian Wolf
    Ophthalmology, Inselspital Bern, Bern, Switzerland
  • Andreas Ebneter
    Ophthalmology, Inselspital Bern, Bern, Switzerland
  • Martin Zinkernagel
    Ophthalmology, Inselspital Bern, Bern, Switzerland
  • Footnotes
    Commercial Relationships   Helena Zimmermann, None; Despina Kokona, None; Sebastian Wolf, None; Andreas Ebneter, None; Martin Zinkernagel, None
  • Footnotes
    Support  Swiss National Science Foundation (#320030_156019)
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2201. doi:
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    • Get Citation

      Helena Zimmermann, Despina Kokona, Sebastian Wolf, Andreas Ebneter, Martin Zinkernagel; Retinal vasculature in mice: A comparison of optical coherence tomography angiography and confocal scanning laser microscopy. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2201.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Optical coherence tomography angiography (OCT-A) allows non-invasive visualization of retinal vessels in vivo. OCT-A was used to characterize the vascular network of the mouse retina and was compared to histology.

Methods : A Spectralis OCT-A prototype operating at 70 kHz was used to investigate the vascular network in BALB/c and C3A.Cg-Pde6b+Prph2Rd2/J mice. Data were compared to confocal microscopy of retinal flat-mounts stained with isolectin IB4. For quantitative analysis the free NCI’s AngioTool software was used. Vessel density, the number of vessel junctions and endpoints were measured and compared between the imaging modalities.

Results : Vasculature in two different retinal planes was identified and quantified. While the superficial vascular plexus showed similar features (e.g. vessel density) when measured with OCT-A or confocal scanning laser microscopy, the vessel density in the deep vascular network measured by OCT-A was significantly higher than with histology (p = 0.0166). The superficial capillary network in dystrophic mice (C3A.Cg-Pde6b+Prph2Rd2/J) seemed to remain unaltered, showing similar features as in BALB/c mice, whereas the deep capillary network was absent probably due to degeneration of the outer retinal layers.

Conclusions : OCT-A is a relatively novel imaging method that can be applied in small animal models and will be useful to monitor changes in the vascular network in experimental settings.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

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