September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Non invasive label free hyperspectral method to assess corneal cross-linking efficacy
Author Affiliations & Notes
  • Giuseppe Lombardo
    IPCF, CNR, Messina, Italy
  • Valentina Villari
    IPCF, CNR, Messina, Italy
  • Norberto Liborio Micali
    IPCF, CNR, Messina, Italy
  • Nancy Leone
    IPCF, CNR, Messina, Italy
  • Marco Lombardo
    IRCCS Fondazione G.B. Bietti, Rome, Italy
  • Footnotes
    Commercial Relationships   Giuseppe Lombardo, Vision Engineering Italy srl (P); Valentina Villari, Vision Engineering Italy srl (P); Norberto Liborio Micali, Vision Engineering Italy srl (P); Nancy Leone, None; Marco Lombardo, Vision Engineering Italy srl (P)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2893. doi:
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    • Get Citation

      Giuseppe Lombardo, Valentina Villari, Norberto Liborio Micali, Nancy Leone, Marco Lombardo; Non invasive label free hyperspectral method to assess corneal cross-linking efficacy. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2893.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To present a novel, non invasive, optical method for assessing the concentration of stromal riboflavin in riboflavin/UV-A corneal cross-linking in order to determine treatment efficacy.

Methods : Eight human donor corneas were de-epithelialized and soaked with 20% dextran-enriched 0.1% riboflavin. Four tissues were then irradiated using 3 mW/cm2 UV-A device for 30 minutes and the other four using 10 mW/cm2 UV-A device for 9 minutes. During treatment, corneal specimens were imaged using a novel optical method in order to collect their hyperspectral response in real time. A calibrated RGB camera recorded the fluorescence and the diffuse reflectance images from each tissue and a processor elaborated the information in order to evaluate the stromal riboflavin concentration both during stromal soaking and UV-A irradiation of the cornea. Immediately after treatment, the elastic modulus of the anterior stroma in each specimen was probed by means of atomic force microscopy.

Results : After stromal soaking, the concentration of riboflavin in the stroma showed consistent results between tissues (0.016% ± 0.003%). During UV-A irradiation, the stromal riboflavin concentration decreased non linearly over time, then reaching the minimum value of 0.006% ± 0.002% at the end of treatment. A regression model was developed to correlate the stromal riboflavin consumption with the increase of corneal stiffness. The results of the model were highly significant (R = 0.79; P = 0.03).

Conclusions : The present label-free optical method shows to be reliable for the assessment of stromal riboflavin concentration during corneal cross-linking. The method is an active monitoring solution of the stromal riboflavin consumption during corneal UV-A irradiation, which provides an estimation of treatment efficacy in real-time.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

During corneal cross-linking, the corneal tissue is imaged by a RGB camera. The hyperspectral response of the cornea is evaluated in a region of interest (green circle) in order to assess the stromal riboflavin concentration and the induced stiffness.

During corneal cross-linking, the corneal tissue is imaged by a RGB camera. The hyperspectral response of the cornea is evaluated in a region of interest (green circle) in order to assess the stromal riboflavin concentration and the induced stiffness.

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