September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Establishment of retinal ganglion cell-specific gene recombination murine models using tet system
Author Affiliations & Notes
  • Yusaku Katada
    Laboratory of Photobiology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Maki Miyauchi
    Laboratory of Photobiology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Yukihiro Miwa
    Laboratory of Photobiology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Xiaoyan Jiang
    Laboratory of Photobiology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Kiwako Mori
    Laboratory of Photobiology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Hidemasa Torii
    Laboratory of Photobiology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Kenji F Tanaka
    Department of Neuropsychiatry, Keio University School of Medicine , Tokyo, Japan
  • Kazuo Tsubota
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Toshihide Kurihara
    Laboratory of Photobiology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships   Yusaku Katada, None; Maki Miyauchi, None; Yukihiro Miwa, None; Xiaoyan Jiang, None; Kiwako Mori, None; Hidemasa Torii, None; Kenji Tanaka, None; Kazuo Tsubota, None; Toshihide Kurihara, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 747. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Yusaku Katada, Maki Miyauchi, Yukihiro Miwa, Xiaoyan Jiang, Kiwako Mori, Hidemasa Torii, Kenji F Tanaka, Kazuo Tsubota, Toshihide Kurihara; Establishment of retinal ganglion cell-specific gene recombination murine models using tet system. Invest. Ophthalmol. Vis. Sci. 2016;57(12):747.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : It is generally difficult to obtain an efficient gene recombination in a specifically targeted cell population in order to generate conditional transgenic mice. To establish both highly specific and sufficient gene recombination simultaneously, we utilize a tetracycline-controllable gene expression system (tet system, Fig. 1) in which the amount of gene expression have been much improved and previously reported (KENGE-tet system). In the present study we establish retinal ganglion cell (RGC)-specific gene recombination murine models with high efficiency using this system.

Methods : We employed two different mouse lines which express the gene encoding tetracycline transactivation (tTA) protein under the control of a cell-type-specific promoter, muscarinic acetylcholine receptor M4 or serotonin receptor 5B control region. Those mice were further crossed with another transgenic mouse line which contains the yellow cameleon (YC) fluorescent gene connected into the downstream of the tet operator (tetO) promoter. The YC gene expression was induced only by the presence of tTA protein in the double transgenic mice (M4-tTA::tetO-YC or 5B-tTA::tetO-YC). The expression of YC was observed in the double transgenic mouse retina with a fluorescence microscope.

Results : In the M4-tTA::tetO-YC mouse retina we identified the expression of YC mainly in RGC and in some population of amacrine cells, whereas RGC specific expression of YC was observed in the 5B-tTA::tetO-YC mouse retina .

Conclusions : Using the tet system, RGC was visualized and the cell-type-specific gene recombination was confirmed. These results suggest that the current tetO-based system is useful to modify gene expression specifically in RGC.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

 

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×