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Kaspar Schürch, Stephen H Tsang, Winston Lee, Katherine Boudreault, Tobias Duncker, Russell L Woods, Francois C Delori, Janet R Sparrow; Quantifying Fundus Autofluorescence Rings in Patients with Retinitis Pigmentosa. Invest. Ophthalmol. Vis. Sci. 2016;57(12):148.
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© 2017 Association for Research in Vision and Ophthalmology.
Quantitative fundus autofluorescence (qAF) enables measurement of fundus AF intensities amongst groups of individuals. We applied the qAF approach to study the high autofluorescent rings that are often a feature of fundus autofluorescence (AF) images obtained from patients with retinitis pigmentosa (RP).
Ten (10) RP patients (age 15 to 56 years; total 17 eyes) having clearly defined autofluorescent rings, clear lenses and no floaters were selected for study. Inheritance was autosomal dominant in 3 patients and autosomal recessive in the others. AF images (30°, 488 nm excitation) were acquired with a confocal scanning laser ophthalmoscope equipped with an internal fluorescent reference. After exclusion of the vessels, gray level intensities in rectangular regions of interest (ROI) (2395 ± 930 pixels) over and outside the ring were obtained in the superior, temporal and inferior aspect of each ring. qAF-units were calculated. Control values consisted of previously published data from age-similar healthy subjects with no family history of retinal dystrophy. qAF values are reported relative to the 95% confidence interval (CI) for the same retinal location in age-matched healthy eyes.
qAF values outside and over the rings could be higher, similar or lower than qAF values at the same position in age-similar healthy eyes. Seven (7) eyes were found to have higher qAF-units in all 3 quadrants over of the ring compared to qAF values at the same location in healthy age-matched eyes. Additionally, in 2, 1 and 0 eyes qAF was only higher temporally, inferiorly and superiorly, respectively. Thus in 24/51 eyes (47%) qAF in the ROI over the ring was higher than in healthy eyes. One eye had qAF-units below the 95%CI outside the ring, with values over the ring being within the normal range in all 3 quadrants.
Although the qAF values associated with AF rings in the RP patients varied, in 47% of cases, qAF over the ring was outside the 95% CI for age-matched healthy eyes. The high qAF indicates that increased fluorophore production may be a factor in the formation of the rings.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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