September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Resvega in the regulation of autophagy
Author Affiliations & Notes
  • Celine Olmiere
    Ophthalmology, Laboratoires Thea, Clermont-Ferrand, France
  • Ali Koskela
    Department of Ophthalmology, University of Eastern Finland, Kuopio, Finland
  • Mika Reinisalo
    School of Pharmacy, University of Eastern Finland, Kuopio, Finland
  • Reijo Karjalainen
    Department of Biology, University of Eastern Finland, Kuopio, Finland
  • Kai Kaarniranta
    Department of Ophthalmology, University of Eastern Finland, Kuopio, Finland
  • Footnotes
    Commercial Relationships   Celine Olmiere, Laboratoires THEA (E); Ali Koskela, None; Mika Reinisalo, None; Reijo Karjalainen, None; Kai Kaarniranta, Laboratoires THEA (C)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 182. doi:
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    • Get Citation

      Celine Olmiere, Ali Koskela, Mika Reinisalo, Reijo Karjalainen, Kai Kaarniranta; Resvega in the regulation of autophagy. Invest. Ophthalmol. Vis. Sci. 2016;57(12):182.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Purpose : Impaired autophagic and proteasomal cleansing has been documented in retinal pigment epithelial (RPE) cells and age-related macular degeneration (AMD) pathology. Omega fatty acids and resveratrol has been shown to be cytoprotective in RPE cells. We examined effects of commercial Resvega on the regulation of autophagy and cytoprotection in ARPE-19 cells under proteasome inhibition.

Methods : Protein aggregation was induced with 1 µM MG-132. Resvega, which includes vitamin C 240 mg, E 30 mg, zinc 12,5 mg, copper 1 mg, omega-3 665 mg, lutein 10 mg, zeaxanthin 2 mg and resveratrol 30 mg, was used solely and together with proteasome inhibition up to 48 hours.. SQSTM1/p62, MAP1LC3A/LC3, ubiquitin protein conjugates and Hsp70 were analyzed by western blotting (WB). Tandem fluorescently tagged LC3 (GFP-mCherry-LC3) transfection was used to study autophagy flux in fluorescence microscopy.

Results : Inhibition of proteasomes with MG-132 upregulated ubiquitin protein conjugates and Hsp70 and autophagy markers SQSTM1/p62 and MAP1LC3A/LC3 detected in WB. Simultaneous treatment with MG-132 and Resvega corresponding 15 and 25 µM resveratrol concentration highly increased amount of LC3-II, but decreased SQSTM1/p62 and Hsp70. Moreover, Resvega provided a clear cytoprotection under proteasome inhibition. Fluorescence microscopy showed increased autophagy flux with GFP-mCherry-LC3 Resvega treatment.

Conclusions : Resvega has a potential to further studies in the prevention of RPE damage and AMD.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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