September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Impairment of development of orbicularis oculi muscle and meibomian gland in Six1/4 double knockout mice
Author Affiliations & Notes
  • Shin Mizoguchi
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • Keiko Ikeda
    Division of Biology, Hyogo College of Medicine, Hyogo, Japan
  • Yilu Xie
    Gavin Herbert Eye Institute, University of California, Irvine, California, United States
  • Yuka Okada
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • James V Jester
    Gavin Herbert Eye Institute, University of California, Irvine, California, United States
  • Shizuya Saika
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • Footnotes
    Commercial Relationships   Shin Mizoguchi, None; Keiko Ikeda, None; Yilu Xie, None; Yuka Okada, None; James Jester, None; Shizuya Saika, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 225. doi:
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      Shin Mizoguchi, Keiko Ikeda, Yilu Xie, Yuka Okada, James V Jester, Shizuya Saika; Impairment of development of orbicularis oculi muscle and meibomian gland in Six1/4 double knockout mice. Invest. Ophthalmol. Vis. Sci. 2016;57(12):225.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To examine the effects on embryonic development of eyelids and orbicularis oculi muscles and meibomian glands in Six1 and Six4 (Six1/4) double knockout mice.

Methods : Six1/4-double knockout (dKO) and littermate wild-type (WT) embryos were used. Paraffin sections or whole-mounted specimens of eyelids were examined at embryonic day (E)15.5 (n = 4), E16.5 (n = 4) and E17.5 (n = 4). Paraffin sections were processed for hematoxylin and eosin (HE) staining or immunohistochemistry for a-smooth muscle actin. Whole-mount eyelid specimens were processed for phalloidin staining and observed under confocal microscopy.

Results : A dKO mouse died just after birth due to a breathing problem. At and after E16.5 the morphogenesis of eyelid was found to be severely impaired in dKO embryos. Histology and immunohistochemistry showed disturbance of the development of meibomian glands and orbicularis oculi muscles in dKO embryos. Eye including intraocular structures were well formed. Confocal microscopy observation of eyelid samples with phalloidin staining nicely showed severely impaired development of orbicularis oculi muscles in dKO embryos at E16.5 and E17.5 as compared with WT embryos.
A dKO mouse died just after birth due to a breathing problem. At and after E16.5 the morphogenesis of eyelid was found to be severely impaired in dKO embryos. Histology and immunohistochemistry showed disturbance of the development of meibomian glands and orbicularis oculi muscles in dKO embryos. Eye including intraocular structures were well formed. Confocal microscopy observation of eyelid samples with phalloidin staining nicely showed severely impaired development of orbicularis oculi muscles in dKO embryos at E16.5 and E17.5 as compared with WT embryos.

Conclusions : Deletion of both Six1/4 impaired embryonic development of eyelids and meibomian glands, as well as orbicularis oculi muscles, in mice. Because Six1/4 are known to be involved in growth of striated muscle, morphogenesis of orbicularis oculi muscle might be critical in eyelid development.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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