September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
AGING EFFECTS ON CORNEAL COLD THERMORECEPTOR NEURONS AND CORNEAL NERVES ARE RELATED WITH CHANGES IN BASAL TEARING AND BLINKING RATES
Author Affiliations & Notes
  • Almudena Íñigo-Portugués
    Ocular Surface, Instituto Universitario Fernández-Vega, Oviedo, Asturias, Spain
  • Omar Gonzalez Gonzalez
    Ocular Surface, Instituto Universitario Fernández-Vega, Oviedo, Asturias, Spain
  • Ignacio Alcalde
    Ocular Surface, Instituto Universitario Fernández-Vega, Oviedo, Asturias, Spain
  • Federico Bech
    Ocular Surface, Instituto Universitario Fernández-Vega, Oviedo, Asturias, Spain
  • Juana Gallar
    Instituto de Neurociencias , San Juan de Alicante, Spain
  • Jesus Merayo-Lloves
    Ocular Surface, Instituto Universitario Fernández-Vega, Oviedo, Asturias, Spain
    Universidad de Oviedo, Oviedo, Spain
  • Carlos Belmonte
    Ocular Surface, Instituto Universitario Fernández-Vega, Oviedo, Asturias, Spain
    Instituto de Neurociencias , San Juan de Alicante, Spain
  • Footnotes
    Commercial Relationships   Almudena Íñigo-Portugués, None; Omar Gonzalez Gonzalez, None; Ignacio Alcalde, None; Federico Bech, None; Juana Gallar, None; Jesus Merayo-Lloves, None; Carlos Belmonte, None
  • Footnotes
    Support  SAF2014-54518-C3-1-R; SAF2014-54518-C3-2-R
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 402. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Almudena Íñigo-Portugués, Omar Gonzalez Gonzalez, Ignacio Alcalde, Federico Bech, Juana Gallar, Jesus Merayo-Lloves, Carlos Belmonte; AGING EFFECTS ON CORNEAL COLD THERMORECEPTOR NEURONS AND CORNEAL NERVES ARE RELATED WITH CHANGES IN BASAL TEARING AND BLINKING RATES. Invest. Ophthalmol. Vis. Sci. 2016;57(12):402.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To determine in mice the effects of aging on morphometric, neurochemical and functional parameters of corneal cold sensory fibers and their relation with basal tearing and blinking rates

Methods : Corneas and trigeminal ganglia (TG) obtained from TRPM8BAC-EYFP and TRPM8-deficient mice of different ages (3 to 24 months) were studied. Blinking responses to chemical stimuli (osmolality and menthol) were measured in awake, restrained mice. Basal tearing was measured in anesthetized animals, using phenol red threads. Corneal nerves expressing EYFP protein were identified in whole mount corneas of mice using immunohistochemical techniques. Also, TGs were processed for immunofluorescence techniques against peripherin, NF20, TrkA and CGRP. Extracellular electrical activity of single sensory nerve endings of the corneal surface was recorded in excised and superfused eyes

Results : The density, length and shape of corneal cold subbasal nerve fibers and terminals were altered by age in TRPM8BAC-EYFP mice corneas, resembling those found in young TRPM8-deficient mice. The density of TRPM8 axons in the subbasal plexus and of epithelial nerve terminals decreased with age. In young mice, two types of cold fibers, beaded (~50%) and smooth (~50%) and three types of terminal branches (“complex”, “ramifying” and “simple”) were found. In old mice, smooth axons predominated and their length was higher in comparison with young mice. The proportion of different types of terminals changed with aging and in old animals a new type (“collapsed terminal”) was identified. In the TG, two populations of TRPM8 corneal neurons with different neurochemical signature were identified. Their proportion and molecular pattern changed with age. Functionally, a large number of corneal cold nerve terminals in young mice were low-threshold-with a small number of high threshold terminals. This proportion changed with age, increasing markedly the number of high-threshold cold terminals. In parallel, basal tearing and blinking rates were altered in aged mice

Conclusions : Changes in the morphological, neurochemical and functional properties of corneal cold sensory neurons and their peripheral axons during aging appear to be associated with disturbances in basal tearing and blinking rates

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×