September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Structural and functional changes of lacrimal gland functional unit in CFTR knockout mice
Author Affiliations & Notes
  • Chuanqing Ding
    Ophthalmology, University of Southern California, Arcadia, California, United States
  • Mate Katona
    1st Dept of Internal Medicine, University of Szged, Szeged, Hungary
  • Eszter Vizvari
    Ophthalmology, University of Szeged, Szeged, Hungary
  • Orsolya Berczeli
    Ophthalmology, University of Szeged, Szeged, Hungary
  • Andrea Facsko
    Ophthalmology, University of Szeged, Szeged, Hungary
  • Zoltan Rakonczay Jr
    1st Dept of Internal Medicine, University of Szged, Szeged, Hungary
    Pathophysiology, University of Szeged, Szeged, Hungary
  • Peter Hegyi
    1st Dept of Internal Medicine, University of Szged, Szeged, Hungary
  • Edit Toth-Molnar
    Ophthalmology, University of Szeged, Szeged, Hungary
    Pharmacology and Pharmacotherapy, University of Szeged, Szeged, Hungary
  • Footnotes
    Commercial Relationships   Chuanqing Ding, None; Mate Katona, None; Eszter Vizvari, None; Orsolya Berczeli, None; Andrea Facsko, None; Zoltan Rakonczay Jr, None; Peter Hegyi, None; Edit Toth-Molnar, None
  • Footnotes
    Support  The Webb Foundation; NKFIH NN 115611; NIH/NEI-R01 EY017731
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 430. doi:
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    • Get Citation

      Chuanqing Ding, Mate Katona, Eszter Vizvari, Orsolya Berczeli, Andrea Facsko, Zoltan Rakonczay Jr, Peter Hegyi, Edit Toth-Molnar; Structural and functional changes of lacrimal gland functional unit in CFTR knockout mice. Invest. Ophthalmol. Vis. Sci. 2016;57(12):430.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Lacrimal gland (LG) is the major source of tears, which is mediated by an array of ion transporters/channels. Cystic fibrosis transmembrane conductance regulator (CFTR) plays a critical role in many exocrine glands, but its role in LG secretion is unknown. Availability of transgenic mouse models carrying genetic defects in CFTR allows direct examination of the role of CFTR in LG secretion, and therefore the aim of the present study is to investigate potential structural and functional changes from CFTR knock out (KO) mice, as compared to wild types (WT).

Methods : Mice used throughout the studies were based on the congenic FVB/N background. Morphometric assessment of LG was used to evaluate potential changes of morphology in KO mice. Tear production was measured in anesthetized mice using phenol red impregnated cotton threads, and wetting length were measured. Ocular surface integrity was investigated by applying fluorescein sodium into the conjunctival sac, followed by corneal evaluation by a slit lamp biomicroscope equipped with cobalt-blue light, with the staining assessed by using the 2007 Dry Eye WorkShop (DEWS) grading system. The cornea was divided into five regions and staining was assessed and rated in each region from 0 to 4. The total score from the five regions was recorded and analyzed, with p<0.05 was considered statistically significant.

Results : Morphometric assessment of acinar and ductal lumens did not reveal noticeable differences of LG morphology between KO and WT mice, as contrast to what has been suspected before. Tear secretion from mice at 8-10 weeks were 2.36±0.31 mm/5 min (n=7) in WT mice, and 1.28±0.19 mm/5 min (n=11) in KO mice (p=0.0021). In 14-16 weeks group, tear secretion was 4.68±0.69 mm/5 min (n=9) in WT mice and 2.33±0.68 mm/5 min (n=5) in KO mice (p=0.0027). For those mice at 22-24 weeks, tear secretion was 3.35±0.6 mm/5 min (n=7) in the WT group and 1.37±0.08 mm/5 min (n=4) in KO group (p=0.00007). Corneal staining score was 1.5±1.16 in WT mice (n=6) and 9.16±1.83 in KO mice (n=6, p=0.00008) in animals at 8-10 weeks, while in the 22-24 weeks group, the staining was 7.16 ±1.5 in WT mice (n=6) and 15.33±1.66 in KO animals (n=6, p=0.0018).

Conclusions : These data demonstrated significant decreases in tear secretion and defects in ocular surface integrity in KO mice, suggesting the key role CFTR may play in LG secretion and the maintenance of ocular surface integrity.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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