September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Effects of silk fibroin in a dry eye mouse model
Author Affiliations & Notes
  • Chae Eun Kim
    Ocular Neovascular Disease Research Center, Inje University Busan Paik Hospital, Busan, Korea (the Republic of)
  • Jihyun Lee
    Ocular Neovascular Disease Research Center, Inje University Busan Paik Hospital, Busan, Korea (the Republic of)
  • So Yeon Choi
    Ocular Neovascular Disease Research Center, Inje University Busan Paik Hospital, Busan, Korea (the Republic of)
  • Min Chae Lee
    Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, Korea (the Republic of)
  • Chan Hum Park
    Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, Korea (the Republic of)
    Departments of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, Chuncheon, Korea (the Republic of)
  • JaeWook Yang
    Ocular Neovascular Disease Research Center, Inje University Busan Paik Hospital, Busan, Korea (the Republic of)
    Department of Ophthalmology, Inje University College of Medicine, Busan, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Chae Eun Kim, None; Jihyun Lee, None; So Yeon Choi, None; Min Chae Lee, None; Chan Hum Park, None; JaeWook Yang, None
  • Footnotes
    Support  This study was supported by a grant from the Korea Healthcare Technology R&D Project of the Ministry of Health and Welfare Affairs, Republic of Korea (Grant Number: HI12C0005).
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 431. doi:
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      Chae Eun Kim, Jihyun Lee, So Yeon Choi, Min Chae Lee, Chan Hum Park, JaeWook Yang; Effects of silk fibroin in a dry eye mouse model. Invest. Ophthalmol. Vis. Sci. 2016;57(12):431.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigated the effects of silk fibroin on the cornea, conjunctiva, and lacrimal gland in a dry eye mouse model.

Methods : The experimental dry eye mouse model was subcutaneous injections of scopolamine (muscarinic receptor blocker) and exposure to an air draft at an ambient humidity of 30-40% for 18 hours per day for 10 days on the 12- to 16-week-old NOD.B10.H2b mice. Tear volume and corneal smoothness were measured at 3, 5, 7 and 10 days after the instillation of phosphate buffered saline (PBS group) or silk fibroin (Silk group; 1 or 5 mg/ml). The corneal detachment and conjunctival goblet cell density were sectioned and stained with hematoxylin and eosin (H&E) and periodic acid Schiff (PAS) in the cornea and conjunctiva. The expression of inflammatory markers (i.e., TNF-α, MMP-2, MMP-9, ICAM-1, and VCAM-1) was detected by immunohistochemistry in the lacrimal gland. All data were statistically processed using the SPSS version 18.0.

Results : The Silk group (1 or 5 mg/ml), tear production was increased by up to 2.1-fold, and corneal smoothness was improved to 46.2% and 53.8%, respectively, compared to the PBS group (P < 0.05). The detachment of the corneal epithelial cells was reduced by 90.9% in the silk group compared to the PBS group, and the conjunctival goblet cell density was significantly recovered to 1.4-fold and 1.7-fold, respectively, compared to the PBS group (P < 0.05). The expression of inflammatory factors was decreased in the lacrimal gland of the silk group compared to the PBS group.

Conclusions : These results show that silk fibroin improved on the cornea, conjunctiva, and lacrimal gland in the dry eye mouse model. These findings suggest that silk fibroin has anti-inflammatory effects in experimental model of dry eye.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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