September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
aB-Crystallin expression in Alzheimer’s Disease brain in neuritic and diffuse plaques
Author Affiliations & Notes
  • Juliet A Moncaster
    Psychiatry, Boston University, Boston, Massachusetts, United States
  • Rajendra K Gangalum
    Ophthalmology, Jules Stein Eye Institute UCLA, Los Angeles, California, United States
  • Olga Minaeva
    Biomedical Engineering, Boston University, Boston, Massachusetts, United States
  • John C Voss
    University of California-Davis, Davis, California, United States
  • Dean Hartley
    Rush University, Chicago, Illinois, United States
  • Suraj P Bhat
    Ophthalmology, Jules Stein Eye Institute UCLA, Los Angeles, California, United States
  • Carmela Abraham
    Alzheimer's Disease Center, Boston University, Boston, Massachusetts, United States
  • Bertrand R Huber
    Alzheimer's Disease Center, Boston University, Boston, Massachusetts, United States
  • Patric K Stanton
    New York Medical College, Valhalla, New York, United States
  • Lee E Goldstein
    Psychiatry, Boston University, Boston, Massachusetts, United States
    Alzheimer's Disease Center, Boston University, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Juliet Moncaster, None; Rajendra Gangalum, None; Olga Minaeva, None; John Voss, None; Dean Hartley, None; Suraj Bhat, None; Carmela Abraham, None; Bertrand Huber, None; Patric Stanton, None; Lee Goldstein, Cognoptix (P)
  • Footnotes
    Support  NIH (NIGMS, NIA), Alzheimer’s Association, American Health Assistance Foundation, Cure Alzheimer’s Foundation (CAF), Boston University Alzheimer’s Disease Center, Anonymous Foundation
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 730. doi:
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    • Get Citation

      Juliet A Moncaster, Rajendra K Gangalum, Olga Minaeva, John C Voss, Dean Hartley, Suraj P Bhat, Carmela Abraham, Bertrand R Huber, Patric K Stanton, Lee E Goldstein; aB-Crystallin expression in Alzheimer’s Disease brain in neuritic and diffuse plaques. Invest. Ophthalmol. Vis. Sci. 2016;57(12):730.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Alzheimer’s disease plaques in the brain are composed of amyloid-β (Aβ) and also contain low molecular weight chaperones including αB-crystallin. We investigated where αB-crystallin is expressed particularly in neuritic versus diffuse plaques and how this may impact AD pathology through interaction with Aβ.

Methods : Immunohistofluorescence, confocal microscopy, immunogold electron microscopy, SDS-page and immunoblotting, spectral reflectance imaging biosensor, electrophysiology, cell and organotypic slice culture.

Results : Immunohistochemical analysis of human Alzheimer’s disease brain revealed co-localization of human Aβ and human αB-crystallin in neuritic and diffuse plaques. Immunohistochemistry and immunoblotting analysis demonstrated that the αB-crystallin is expressed and secreted from astrocytes in close proximity to the plaques and affects Aβ fibrillogenesis and neurotoxcicity.

Conclusions : Our data show that αB-crystallin is expressed in Alzheimer’s Disease human brain in neuritic and diffuse plaques and interacts with Aβ. Our results indicate that αB-crystallin plays an antagonistic pleiotropy role that may be critical to the pathogenesis of Alzheimer’s disease.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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