September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
In Vivo Assessment of Molecular Aging by Quasi-Elastic Light Scattering in the Human Lens
Author Affiliations & Notes
  • Olga Minaeva
    Molecular Aging and Development Laboratory, Department of Psychiatry, Boston University School of Medicine, Boston, Massachusetts, United States
    Department of Biomedical Engineering, Boston University, Boston, Massachusetts, United States
  • Srikant Sarangi
    Molecular Aging and Development Laboratory, Department of Psychiatry, Boston University School of Medicine, Boston, Massachusetts, United States
    Department of Biomedical Engineering, Boston University, Boston, Massachusetts, United States
  • Danielle M. Ledoux
    Department of Ophthalmology, Boston Children’s Hospital, Boston, Massachusetts, United States
    Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States
  • Juliet A Moncaster
    Molecular Aging and Development Laboratory, Department of Psychiatry, Boston University School of Medicine, Boston, Massachusetts, United States
  • Caitlin A. Rook
    Department of Ophthalmology, Boston Children’s Hospital, Boston, Massachusetts, United States
  • Maria Ericsson
    Electron Microscopy Facility, Harvard Medical School, Boston, Massachusetts, United States
  • Yorghos Tripodis
    Department of Biostatistics, Boston University School of Public Health, Boston, Massachusetts, United States
  • John I Clark
    Department of Biological Structure, University of Washington, Seattle, Washington, United States
  • Rudolph E. Tanzi
    Department of Neurology, Harvard Medical School, Boston, Massachusetts, United States
    Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts, United States
  • David G Hunter
    Department of Ophthalmology, Boston Children’s Hospital, Boston, Massachusetts, United States
    Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States
  • Lee E Goldstein
    Molecular Aging and Development Laboratory, Department of Psychiatry, Boston University School of Medicine, Boston, Massachusetts, United States
    Boston University Alzheimer’s Disease Center, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Olga Minaeva, None; Srikant Sarangi, None; Danielle Ledoux, None; Juliet Moncaster, None; Caitlin Rook, None; Maria Ericsson, None; Yorghos Tripodis, None; John Clark, None; Rudolph Tanzi, None; David Hunter, None; Lee Goldstein, None
  • Footnotes
    Support  Massachusetts Lions Eye Research Fund and the Children’s Hospital Ophthalmology Foundation, Boston, MA
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 735. doi:
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      Olga Minaeva, Srikant Sarangi, Danielle M. Ledoux, Juliet A Moncaster, Caitlin A. Rook, Maria Ericsson, Yorghos Tripodis, John I Clark, Rudolph E. Tanzi, David G Hunter, Lee E Goldstein; In Vivo Assessment of Molecular Aging by Quasi-Elastic Light Scattering in the Human Lens. Invest. Ophthalmol. Vis. Sci. 2016;57(12):735.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Crystallin proteins comprise ~90% of lens protein in mature lens fiber cells, do not undergo turnover, and thus remain extant throughout life. Crystallins undergo various post-translational modifications during aging. We hypothesize that cumulative alterations in the lens may constitute an in vivo biomarker of molecular aging for the human body.

Methods : Quasi-elastic light scattering (QLS) has been used to study the aggregation of lens proteins. Here we use QLS to measure changes in human lens proteins as a function of time in vitro and aging in vivo. We investigated changes in light scattering in vivo from the lenses of 34 healthy human subjects without history of eye disease (18 males, 16 females). Measurements were acquired in the nucleus along the optical axis at a predetermined distance from the anterior capsule of the lens.

Results : QLS detected time-dependent changes in scattering intensity, hydrodynamic radius, and supramolecular order of human lens proteins during long-term incubation (~1 yr) and in response to oxidation in vitro. Changes in the time-dependent QLS signal observed in vitro mirror changes in the age-dependent QLS signal detected in lenses of healthy humans across a wide age range (5 to 61 years).

Conclusions : QLS assessment of long-lived protein in the human lens can provide a practical noninvasive technique for objective evaluation of molecular aging in vivo.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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