September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Human Corneal Limbal Stromal Cells provide a source of Mesenchymal Stem Cells
Author Affiliations & Notes
  • Ilham Putra
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Gaurav Agnihotri
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Samaneh Ghassemi
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Judy Hamouie
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Asha Tadepalli
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Xiang Shen
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Peiman Hematti
    Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States
  • Medi Eslani
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Ali R Djalilian
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Ilham Putra, None; Gaurav Agnihotri, None; Samaneh Ghassemi, None; Judy Hamouie, None; Asha Tadepalli, None; Xiang Shen, None; Peiman Hematti, None; Medi Eslani, None; Ali Djalilian, None
  • Footnotes
    Support  Supported in part by Clinical Scientist Development Program Award K12EY021475 (ME), R01 EY024349-01A1 (ARD) and Core grant EY01792 from NEI/NIH; MR130543 (ARD) from DoD; and unrestricted grant to the department from RPB.
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 892. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Ilham Putra, Gaurav Agnihotri, Samaneh Ghassemi, Judy Hamouie, Asha Tadepalli, Xiang Shen, Peiman Hematti, Medi Eslani, Ali R Djalilian; Human Corneal Limbal Stromal Cells provide a source of Mesenchymal Stem Cells. Invest. Ophthalmol. Vis. Sci. 2016;57(12):892.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Mesenchymal stem cell (MSC)-based therapy is under investigation as a treatment strategy to promote tissue repair and to modulate the immune system in immune mediated disorders. MSCs have been isolated from many tissues most commonly from the bone marrow or fat. Given the cumbersome procedures for harvesting bone marrow (BM), and to a lesser extent fat, there is a need to look for other tissues for MSC isolation. The International Society for Cellular Therapy (ISCT) has established three criteria for characterizing human MSCs: adherence to plastic, differentiation potential, and the presence or absence of certain cell surface markers. We evaluated whether or not corneal limbal stromal cells (CLSC) met ISCT criteria by comparing them to BM-MSCs.

Methods : BM-MSCs were isolated from healthy human bone marrow donors. CLSC were isolated from human corneal limbus from research cadaver corneas (generously provided by Eversight). Both cell types were cultured in MEM- alpha + 10% fetal bovine serum. They were photographed for morphology at each passage. Cells from passage 4 were used for immunophenotyping using fluorescent-activated cell sorting (BD LSR Fortessa). Their differentiation into the three mesenchymal lineages (adipocytes, osteoblasts, and chondrocytes) was evaluated using staining with LipidTOX™ for fatty acids, Alizarin Red for calcium, and Alcian blue for glycosaminoglycans, respectively. All experiments were repeated with at least five different donors.

Results : CLSCs and BM-MSCs were both able to adhere to the plastic. They had identical morphology. They were positive for the cell surface markers CD90, CD105, CD73 and negative for CD34, CD45, HLA-DR, CD19, and CD11b. After incubation in differentiation media, they were able to differentiate successfully into adipocytes, osteoblasts, and chondrocytes.

Conclusions : Based on ISCT criteria, CLSCs can be considered MSCs. They can be used as a convenient source of MSC for the cell-based therapy as they can be obtained from corneas from eye bank.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×