September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Spatially-resolved proteomics by imaging mass spectrometry of ocular tissues
Author Affiliations & Notes
  • Kevin L Schey
    Vanderbilt University, Nashville, Tennessee, United States
  • Footnotes
    Commercial Relationships   Kevin Schey, None
  • Footnotes
    Support  NIH grant EY013462; NIH grant EY008126; NIH grant GM103391
Investigative Ophthalmology & Visual Science September 2016, Vol.57, No Pagination Specified. doi:
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      Kevin L Schey; Spatially-resolved proteomics by imaging mass spectrometry of ocular tissues. Invest. Ophthalmol. Vis. Sci. 201657(12):.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Presentation Description : Proteomics methods have provided a comprehensive view of protein expression and modification in many tissues including specific ocular tissues; however, the location of protein expression within tissues remains an important, relatively unexplored area. Although immunohistochemical approaches can be used to define protein expression in tissues, this targeted approach requires specific antibodies that are often unavailable, particularly for modified proteins. Imaging mass spectrometry (IMS) produces images based on protein molecular weight and provides spatially-resolved information on hundreds to thousands of proteins in a single experiment. This presentation will describe experimental aspects of protein imaging by IMS including sample preparation, spatial resolution, and data analysis. In addition, specific examples of protein imaging in ocular tissues will be presented along with a view towards future capabilities.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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