September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Mesenchymal stem cells modulate the differentiation of myeloid progenitor cells in corneal inflammation
Author Affiliations & Notes
  • Afsaneh Amouzegar
    Schepens Eye Research Institute , Harvard Medical School, Boston, Massachusetts, United States
  • Sharad Mittal
    Schepens Eye Research Institute , Harvard Medical School, Boston, Massachusetts, United States
  • Sunil Chauhan
    Schepens Eye Research Institute , Harvard Medical School, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Afsaneh Amouzegar, None; Sharad Mittal, None; Sunil Chauhan, None
  • Footnotes
    Support  Department of Defense (W81XWH-15-1-0024) and NIH EY12963
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 1434. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Afsaneh Amouzegar, Sharad Mittal, Sunil Chauhan; Mesenchymal stem cells modulate the differentiation of myeloid progenitor cells in corneal inflammation. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1434.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Mesenchymal stem cells (MSCs) display unique anti-inflammatory and immunomodulatory properties and have potential therapeutic applications in various inflammatory diseases. The purpose of this study was to investigate the effect of MSCs on maturation and differentiation of myeloid progenitor cells in corneal inflammation.

Methods : Mesenchymal stem cells were generated from bone marrow of wild-type C57BL/6 mice, and characterized by flow cytometry for the expression of CD29+SCA1+CD45CD34. Corneal injury was induced by mechanical removal of the corneal epithelium and anterior stroma. For in vitro assays, murine myeloid progenitor cells (CD34+CD14+CD11bLy6GLy6C) were sorted from the spleen using flow cytometry. In order to investigate the effect of MSCs on corneal inflammation, MSCs were intravenously injected to mice with corneal injury. Frequencies of myeloid progenitor cells and CD11b+ cells in the injured cornea were determined after 48 hours. To further investigate immunomodulatory function of MSCs, myeloid progenitors were co-cultured with MSCs in the presence of IFNγ for 72 hours. Myeloid progenitor cell acquisition of CD11b (αM integrin) and their further differentiation into monocytic (Ly6Chi Ly6G) vs. granulocytic (Ly6Clo Ly6G+) lineage of suppressor cells was assessed using flow cytometry.

Results : Systemic administration of MSCs in mice with corneal injury resulted in a 2-fold increase in the frequencies of corneal myeloid progenitor cells, and a significant 50% decrease in the frequencies of corneal CD11b+ cells compared to injured mice without MSC treatment. Myeloid progenitor cells co-cultured with MSCs demonstrated a significant 68% reduction in CD11b (αM integrin) acquisition compared to progenitors cultured without MSCs (4.3±1.6% vs 13.4±4.6%; p<0.001). Interestingly, the majority of these CD11b expressing cells demonstrated a phenotype of monocytic Ly6Chi Ly6G suppressor cells (64.1±5.8%) compared to CD11b+ cells differentiated from progenitors co-cultured without MSCs (23.7±4.7%; p<0.001).

Conclusions : Our data suggest that MSCs modulate corneal inflammation by reducing CD11b+ cell frequencies in the injured cornea, and by promoting differentiation of myeloid progenitor cells toward anti-inflammatory monocytic suppressor cells.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×