September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Immunomodulatory Gene Expression Profile of Corneal-Limbal Versus Bone Marrow derived Mesenchymal Stem Cells
Author Affiliations & Notes
  • Asha Tadepalli
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Medi Eslani
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Judy Hamouie
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Gaurav Agnihotri
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Samaneh Ghassemi
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Ilham Putra
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Neda Afsharkhamseh
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Peiman Hematti
    Division of Hematology/Oncology, Department of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States
  • Ali R Djalilian
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Asha Tadepalli, None; Medi Eslani, None; Judy Hamouie, None; Gaurav Agnihotri, None; Samaneh Ghassemi, None; Ilham Putra, None; Neda Afsharkhamseh, None; Peiman Hematti, None; Ali Djalilian, None
  • Footnotes
    Support  Supported in part by Clinical Scientist Development Program Award K12EY021475 (ME), R01 EY024349-01A1 (ARD) and Core grant EY01792 from NEI/NIH; MR130543 (ARD) from DoD; and unrestricted grant to the department from RPB.
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 1437. doi:
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      Asha Tadepalli, Medi Eslani, Judy Hamouie, Gaurav Agnihotri, Samaneh Ghassemi, Ilham Putra, Neda Afsharkhamseh, Peiman Hematti, Ali R Djalilian; Immunomodulatory Gene Expression Profile of Corneal-Limbal Versus Bone Marrow derived Mesenchymal Stem Cells. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1437.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Purpose : Mesenchymal stem cells (MSCs) are responsible for recruiting and modulating various cells that participate in tissue repair and regeneration. Bone marrow is a frequently used source for MSCs, though they have been isolated from other areas of the body, including the cornea. We compared the gene expression of well-known immunomodulatory factors in the corneal limbal derived MSCs (CL-MSC) versus bone marrow derived MSCs (BM-MSC).

Methods : MSCs were isolated from healthy human bone marrow donors or from human corneal limbus from research corneas generously provided by Eversight eye bank. After reaching to confluency, passage 4 cells were growth factor starved overnight and RNA was extracted, reverse transcribed and analyzed with ΔΔCt method by real-time qPCR. TNF-stimulated gene-6 (TSG6), interleukin-1 receptor antagonist (IL1RN), stanniocalcin-1 (STC1), cyclooxygenase-2 (COX2), peroxisome proliferator-activated receptor gamma (PPAR-γ), and indoleamine-pyrrole 2,3-dioxygenase (IDO) gene expression were studied. All experiments were repeated with at least five different donors.

Results : TSG6 mRNA was expressed 2.9 ± 0.8 folds more in CL-MSCs compared to BM-MSCs (P < 0.001). Likewise, there were 36.8 ± 2.1, 15.2 ± 1.6 and 8.2 ± 2.7 fold increase in STC1, COX2 and PPAR-γ mRNA expression in CL-MSCs compared to BM-MSCs, respectively (p value for each comparison < 0.01). No significant expression of IL1RN and IDO was found in either CL-MSCs or BM-MSCs.

Conclusions : At the mRNA level, CL-MSCs express higher levels of TSG6, STC1 and COX2 compared to BM-MSCs. Further investigation is needed to delineate any differences at the protein and prostaglandin level as well.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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