September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
The Threshold of Light Energy to Eliminate Acanthamoeba
Author Affiliations & Notes
  • Ahmara Gibbons Gibbons Ross
    Ophthalmology, University of Pennsylvania, Philadelphia, Pennsylvania, United States
    Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, United States
  • Regis P Kowalski
    Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, United States
  • Deepinder Dhaliwal
    Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, United States
  • Lisa M Karenchak
    Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, United States
  • Footnotes
    Commercial Relationships   Ahmara Gibbons Ross, None; Regis Kowalski, None; Deepinder Dhaliwal, None; Lisa Karenchak, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 1456. doi:
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      Ahmara Gibbons Gibbons Ross, Regis P Kowalski, Deepinder Dhaliwal, Lisa M Karenchak; The Threshold of Light Energy to Eliminate Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1456.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Acanthamoeba keratitis is a contact lens-related infection that may result in significant visual impairment. Treatment of Acanthamoeba keratitis can be difficult due to a lack of good medical therapy. Acanthamoeba can survive in contact lens storage cases and on the surface of contact lenses for extended periods of time. The aim of this study was to determine the threshold of Ultraviolet energy (UVE) to eliminate Acanthamoeba from contact lens and cases. We hypothesize that UVE with and without riboflavin will eradicate Acanthamoeba while maintaining the clarity of the lens.

Methods : The Stratagene UV Stratalinker 2400 Crosslinker™ was used to administer UVE in pulses to eliminate Acanthamoeba castellanii. Multiple modes of energy delivery were administered in a dose-dependent manner in microjoules/cm2 x 100. Three groups were tested: 1) Acanthamoeba castellanii (106 cysts in saline)exposed to UVE alone; 2) Acanthamoeba castellanii (106 cysts in saline) plus soft contact lens exposed to UVE alone; 3) Acanthamoeba castellanii (106 cysts) plus soft contact lens exposed to UVE in a saline solution of 0.01% Riboflavin. Acanthamoeba castellanii viability was monitored for growth using non-nutrient agar with Enterobacter aerogenes overlay. The soft contact lenses were for evaluated for clarity by spectrophotometry.

Results : A total UVE of 89,991 microjoules/cm2 x 100for a total of 180 min (3 hours) were required to eliminate Acanthamoeba castellanii from all three groups. One large dose in this amount of UVE did not affect the clarity of soft contact lenses in the time-frame of this study.

Conclusions : A very large dose of UVE has the potential to eliminate Acanthamoeba from contact lens and cases. A one time exposure of contact lens to 89,991 microjoules x 100/cm2 UVE may produce minimal damage to soft contact lens.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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