September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Release of moxifloxacin from daily disposable contact lenses using an in vitro eye model: Impact of artificial tear fluid composition and mechanical rubbing
Author Affiliations & Notes
  • Chau-Minh Phan
    School of Optometry and Vision Science, University of Waterloo, Waterloo, Ontario, Canada
  • Magdalena Bajgrowicz
    Material Science and Engineering, Wroclaw University of Technology, Wroclaw, Poland
  • Lakshman N Subbaraman
    School of Optometry and Vision Science, University of Waterloo, Waterloo, Ontario, Canada
  • Lyndon William Jones
    School of Optometry and Vision Science, University of Waterloo, Waterloo, Ontario, Canada
  • Footnotes
    Commercial Relationships   Chau-Minh Phan, None; Magdalena Bajgrowicz, None; Lakshman Subbaraman, None; Lyndon Jones, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 1474. doi:
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      Chau-Minh Phan, Magdalena Bajgrowicz, Lakshman N Subbaraman, Lyndon William Jones; Release of moxifloxacin from daily disposable contact lenses using an in vitro eye model: Impact of artificial tear fluid composition and mechanical rubbing. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1474.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Purpose : The composition of tear fluid and the mechanical rubbing produced by the eye lids are important factors that may influence ocular drug delivery. The aim of this study was to evaluate and compare the release of moxifloxacin from a variety of daily disposable (DD) contact lenses (CLs) under various conditions using a novel in vitro eye model.

Methods : Four commercially available DD conventional hydrogel (CH) CLs (nelfilcon A, omafilcon A, etafilcon A, ocufilcon B) and three silicone hydrogel (SH) CLs (somofilcon A, narafilcon A, delefilcon A) were evaluated. These lenses (n=3 for each lens type) were incubated in moxifloxacin (1mg/mL) for 24 h. The release of the drug was measured using a novel in vitro model in three experimental conditions: (1) phosphate buffered saline (PBS) (2) artificial tear solution (ATS) containing a variety of proteins & lipids and (3) ATS with mechanical rubbing produced by the device. The flow rate was set to 2.1 µL/min (3 mL/24h). The drug was detected using fluorescence spectrophotometry at excitation and emission wavelengths of 296 nm and 471 nm respectively.

Results : Overall, CH CLs had a higher drug release than SH CLs (p<0.05) under all conditions. Typically, a higher drug release was observed in PBS than ATS (p<0.05). For CH, drug release was found to be higher in ATS with rubbing than PBS or ATS (p<0.05). For most lens types, ATS with rubbing produced higher drug release than ATS alone (p<0.05). Total drug release in PBS varied between 27.9±4.0 - 111.3±12.9 µg/lens, in ATS drug release ranged between 7.0±3.2 - 96.2±4.4 µg/lens, and in ATS with rubbing the drug release ranged between 18.1±4.3 - 164.3±15.5 µg/lens. Generally, the release kinetics for all conditions were sustained over the 24 h testing period, and no burst release was observed (p<0.05).

Conclusions : The release of drugs from a CL into ATS is lower when compared to release into PBS. When mechanical rubbing is introduced, the amount of drugs released is increased. These results suggests that drug release from a CL can be significantly influenced by these parameters, and thus sophisticated in vitro models are necessary to adequately model on-eye drug release.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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