September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Analysis of Human Donor Retinas Suggests a Greater Prevalence of Retinal Disease Than Previously Reported in the New Zealand Population
Author Affiliations & Notes
  • Colin Green
    Ophthalmology, Univ of Auckland, Auckland, New Zealand
  • Swathi Kanduri
    Ophthalmology, Univ of Auckland, Auckland, New Zealand
  • Monica L Acosta
    Optometry, University of Auckland, Auckland, New Zealand
  • David Squirrell
    Ophthalmology, Univ of Auckland, Auckland, New Zealand
  • Trevor Sherwin
    Ophthalmology, Univ of Auckland, Auckland, New Zealand
  • Charles NJ McGhee
    Ophthalmology, Univ of Auckland, Auckland, New Zealand
  • Footnotes
    Commercial Relationships   Colin Green, None; Swathi Kanduri, None; Monica Acosta, None; David Squirrell, None; Trevor Sherwin, None; Charles McGhee, None
  • Footnotes
    Support  New Zealand Freemasons Scholarship
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2056. doi:
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      Colin Green, Swathi Kanduri, Monica L Acosta, David Squirrell, Trevor Sherwin, Charles NJ McGhee; Analysis of Human Donor Retinas Suggests a Greater Prevalence of Retinal Disease Than Previously Reported in the New Zealand Population
      . Invest. Ophthalmol. Vis. Sci. 2016;57(12):2056.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Fundus pathology in donor eyes was correlated with cross-sectional Optical Coherence Tomography (OCT) images and histological assessment to determine the prevalence of retinal disease in human donor retinas without the constraints imposed during in vivo clinical imaging.

Methods : A fundus camera and OCT imaging system for small animals was adapted to enable fundus and OCT imaging of entire (including centre and full periphery) post mortem human donor retinas. Retinas from 59 donors (57 retina pairs and two single globes) were imaged in a seven-field imaging format and cross-sectional analysis was done using OCT. To confirm that the signs observed represented true disease incidence analysis of disease markers including, astrocytosis (GFAP expression), hemichannel (Connexin43 expression), Müller cell activation in retina and choroidal leukocytes (vimentin expression), and choroidal macrophages (CD-68 marker) was performed.

Results : Donor ages were 21-89 years with ethnicity 90.5% Caucasian and 9.5% Indian. Gender was 79.3% males, 20.7% females. Retinas from six donors aged 21-60 years of age were unremarkable. Pathological signs were correlated with clinical diagnoses in eyes from 25 donors (ages 45 - 87 years) but signs were also found in eyes from 23 donors (ages 39 to 83 years) with no previously reported clinical diagnosis. Of all these, 5 donors had signs of age related macular degeneration (AMD) and 14 had signs of diabetic retinopathy (DR). Their lesions correlated with OCT signs and histopathology showed signs of inflammation (astrocytes, Müller cell activation, increased Cx43 expression and choroidal inflammation). These data indicate that with over 8% of donors showing signs of AMD and 24% of donors showing signs of DR the incidence of AMD may be 1.7 times higher and DR up to 1.6 times higher than clinically reported.

Conclusions : The detection of pathological signs characteristic of AMD and DR in donors suggests a higher prevalence of posterior segment abnormalities amongst New Zealanders than previously reported.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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