September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Griseofulvin inhibits choroidal neovascularization
Author Affiliations & Notes
  • Timothy William Corson
    Ophthalmology, Indiana University School of Medicine, Indianapolis, Indiana, United States
  • Rania Sulaiman Sulaiman
    Ophthalmology, Indiana University School of Medicine, Indianapolis, Indiana, United States
  • Sameerah Alkhairy
    Ophthalmology, Indiana University School of Medicine, Indianapolis, Indiana, United States
  • Kamna Gupta
    Ophthalmology, Indiana University School of Medicine, Indianapolis, Indiana, United States
  • Halesha Dhurvigere Basavarajappa
    Ophthalmology, Indiana University School of Medicine, Indianapolis, Indiana, United States
  • Footnotes
    Commercial Relationships   Timothy Corson, US Provisional 62/111,149 (P); Rania Sulaiman, None; Sameerah Alkhairy, None; Kamna Gupta, None; Halesha Basavarajappa, US Provisional 62/111,149 (P)
  • Footnotes
    Support  NIH NCATS UL1TR001108, International Retinal Research Foundation, Research to Prevent Blindness, Inc.
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2122. doi:
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    • Get Citation

      Timothy William Corson, Rania Sulaiman Sulaiman, Sameerah Alkhairy, Kamna Gupta, Halesha Dhurvigere Basavarajappa; Griseofulvin inhibits choroidal neovascularization. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2122.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Purpose : Much of the vision loss in the common blinding eye disease wet age-related macular degeneration (AMD) is due to neovascularization of the choroid. No small molecule pharmacotherapies are yet approved for this disease. We previously identified the heme biosynthesis enzyme ferrochelatase as an important mediator of ocular neovascularization; knockdown of ferrochelatase blocked angiogenesis in vitro and in the murine laser-induced choroidal neovascularization (L-CNV) model. Small molecule inhibition of ferrochelatase is thus an appealing approach for impeding neovascularization. Excitingly, the FDA-approved antifungal drug griseofulvin inhibits ferrochelatase as an off-target effect. Hence, here we sought to investigate if griseofulvin can block angiogenesis.

Methods : The anti-angiogenic effects of griseofulvin and its active metabolite, N-methylprotoporphyrin (NMPP), were tested in vitro using proliferation, scratch-wound migration, and tube formation assays with human retinal endothelial cells. The choroidal sprouting assay assessed griseofulvin’s effects on choroidal cells ex vivo. The murine L-CNV model was used to test the in vivo anti-angiogenic potential of griseofulvin, delivered intravitreally or orally. L-CNV was analyzed both in vivo by optical coherence tomography, and ex vivo by confocal microscopy.

Results : Both griseofulvin and NMPP dose-dependently inhibited proliferation, migration, and tube formation of retinal endothelial cells, without obvious toxicity at effective concentrations. Griseofulvin at 50 µM or higher profoundly suppressed choroidal sprouting. In the L-CNV model, griseofulvin dose-dependently decreased choroidal neovascularization. Intravitreally, 50 µM and 100 µM griseofulvin reduced CNV volume by 28 and 67%, respectively. Similarly, 0.5% and 1% (w/w) griseofulvin delivered in food were also efficacious, reducing CNV volume by 33% and 42% (all p<0.05, ANOVA with Tukey’s post hoc tests).

Conclusions : Griseofulvin at clinically achievable concentrations has significant anti-angiogenic potential in the eye. Since oral griseofulvin is already approved for human use and well tolerated during long-term administration, these findings could progress rapidly toward human trials, with potential benefit for the sight of wet AMD patients and applicability to other ocular neovascular diseases.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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