September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Optical coherence tomography angiography (OCT-A) in an animal model for laser-induced choroidal neovascularization
Author Affiliations & Notes
  • Johanna Meyer
    Department of Ophthalmology, University of Bonn, Bonn, Germany
  • Petra Fang
    Department of Ophthalmology, University of Bonn, Bonn, Germany
  • Tim Krohne
    Department of Ophthalmology, University of Bonn, Bonn, Germany
  • Frank G Holz
    Department of Ophthalmology, University of Bonn, Bonn, Germany
  • Steffen Schmitz-Valckenberg
    Department of Ophthalmology, University of Bonn, Bonn, Germany
  • Footnotes
    Commercial Relationships   Johanna Meyer, Carl-Zeiss Meditec AG (F), Heidelberg Engineering (F); Petra Fang, Heidelberg Engineering (F); Tim Krohne, Alcon (F), Bayer (R), Heidelberg Engineering (F), Heidelberg Engineering (R), Novartis (F), Novartis (R); Frank Holz, Alcon (C), Allergan (C), Bayer (C), Carl Zeiss Meditec AG (F), Genentech (C), Heidelberg Engineering (C), Merz (F), Novartis (C), Optos (F), Roche (C); Steffen Schmitz-Valckenberg, Alcon (F), Alcon (C), Allergan (F), Bayer (F), Bayer (R), Carl Zeiss Meditec AG (F), Formycon (F), Genentech (F), Heidelberg Engineering (F), Heidelberg Engineering (R), Optos (F)
  • Footnotes
    Support  German Ministry of Education and Research (BMBF), FKZ 13N10349
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2203. doi:
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    • Get Citation

      Johanna Meyer, Petra Fang, Tim Krohne, Frank G Holz, Steffen Schmitz-Valckenberg; Optical coherence tomography angiography (OCT-A) in an animal model for laser-induced choroidal neovascularization. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2203.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Optical coherence tomography angiography (OCT-A) is a new diagnostic imaging modality for noninvasive three-dimensional visualization of retinal and choroidal vascular structures without fluorescent dye injection. We compared OCT-A with conventional fluorescein angiography in an animal model of laser-induced choroidal neovascularization (CNV).

Methods : Dark Agouti rats underwent argon laser photocoagulation to induce CNV. In-vivo imaging using combined confocal scanner laser ophthalmoscopy (cSLO) and OCT-A imaging (Heidelberg Spectralis, Heidelberg Engineering, Germany) was performed before and directly after laser treatment as well as at day 3, 7, 14 and 21. OCT-A en-face images were compared to cSLO images obtained by conventional fluorescein angiography (FA). Post-mortem analysis included immunohistochemistry of retinal and choroidal blood vessels.

Results : High-resolution non-invasive imaging of normal superficial, intermediate and deep retinal plexus as well as choroidal blood vessels in rats by OCT-A was possible. Within laser lesions, signs of CNV formation occurred at day 7 with progression in size and number of small vessels until day 21. Development of CNV was associated with localized dye leakage on FA. Compared with OCT-A, invasive FA imaging did not allow for a detailed visualization of the formation of new small vessels within laser lesions.

Conclusions : This study demonstrates that in vivo OCT-A imaging can be performed in small animals like rats. Detailed and high-contrast images of the retinal and choroidal vascular plexus can be visualized without invasive dye injection. OCT-A imaging may allow for a more precise, spatial analysis of new blood vessel formation in CNV animal models as compared with conventional FA.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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