September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Viral double-strand RNA increases the membrane-associated mucin mRNA expression in immortalized corneal and conjunctival epithelium
Author Affiliations & Notes
  • Yuriko Ban
    Ophthalmology, Nantan General Hospital, Nantan, Kyoto, Japan
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Tomoyo Fukuyama
    Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan
  • Chie Sotozono
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Shigeru Kinoshita
    Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Footnotes
    Commercial Relationships   Yuriko Ban, None; Tomoyo Fukuyama, None; Chie Sotozono, None; Shigeru Kinoshita, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, No Pagination Specified. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Yuriko Ban, Tomoyo Fukuyama, Chie Sotozono, Shigeru Kinoshita; Viral double-strand RNA increases the membrane-associated mucin mRNA expression in immortalized corneal and conjunctival epithelium. Invest. Ophthalmol. Vis. Sci. 201657(12):.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate the response to polyinosinic:polycytidylic acid [Poly(I:C)], an analog of viral double-stranded RNA produced during viral replication, in the membrane-associated mucin mRNA expression in immortalized corneal and conjunctival epithelium.

Methods : Immortalized corneal (HCLE) and conjunctival epithelial cells (HCjE) were cultured on 12-mm Transwell filters at a density of 4x105 cells/cm2. (n=12) The cultured cells were then stimulated with 25μg/ml of Poly(I:C). Transepithelial electrical resistance (TER) was then measured using endohm electrodes (World Precision Instruments, LTD.). After 6, 12, and 24 hours of exposure to Poly(I:C), the expressions of membrane-associated mucins (MUC1, MUC4, and MUC16) mRNA were analyzed by real-time polymerase chain reaction.

Results : Poly(I:C) challenge increased the TER in a time-dependent manner (HCLE; 0 hours: 233.3±12.0 Ohm・cm2, 6 hours: 335.7±15.9 Ohm・cm2 , 12 hours: 357.4±15.9 Ohm・cm2, and 24 hours: 443.0±17.7 Ohm・cm2, and HCjE; 0 hours: 160.0±16.7 Ohm・cm2, 6 hours: 237.7±27.6 Ohm・cm2 , 12 hours: 262.0±34.7 Ohm・cm2, and 24 hours: 314.7±9.9 Ohm・cm2 ). After 6 hours of Poly(I:C) exposure, no change was observed in all 3 membrane-associated mucin mRNAs. After 12 hours and 24 hours, membrane-associated mucins mRNA were increased.

Conclusions : The findings of this study show that Poly(I:C) challenge, which mimics a viral infection, increased the barrier function of ocular surface epithelia by tightening the tight junction and also by increasing the membrane-associated mucin expression. Thus, we theorize that the increased barrier function must be a kind of host defense reaction to viral infection.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×