September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Interrelationship of primary virus replication in the eye, level of latency and time to reactivate in the trigeminal ganglia (TG) of latently infected mice
Author Affiliations & Notes
  • Homayon Ghiasi
    Surgery-Ophthalmology, Cedars-Sinai Medical Center, Los Angeles, California, United States
  • Kevin Mott
    Surgery-Ophthalmology, Cedars-Sinai Medical Center, Los Angeles, California, United States
  • Sariah Allen
    Surgery-Ophthalmology, Cedars-Sinai Medical Center, Los Angeles, California, United States
  • Yasamin Ghiasi
    USC, Los Angels , California, United States
  • Terrence Town
    USC, Los Angels , California, United States
  • Steven Wechsler
    UCI, Irvine, California, United States
  • Harry Matundan
    Surgery-Ophthalmology, Cedars-Sinai Medical Center, Los Angeles, California, United States
  • Footnotes
    Commercial Relationships   Homayon Ghiasi, None; Kevin Mott, None; Sariah Allen, None; Yasamin Ghiasi, None; Terrence Town, None; Steven Wechsler, None; Harry Matundan, None
  • Footnotes
    Support  This study was supported by Public Health Service NIH grants RO1EY024649 and RO1EY013615.
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2334. doi:
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      Homayon Ghiasi, Kevin Mott, Sariah Allen, Yasamin Ghiasi, Terrence Town, Steven Wechsler, Harry Matundan; Interrelationship of primary virus replication in the eye, level of latency and time to reactivate in the trigeminal ganglia (TG) of latently infected mice. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2334.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Purpose : Very little is known regarding the inter-relationship between primary virus replication in the eye, the level of latency in the TG and the time to reactive of the mouse model. This study was designed to answers these questions.

Methods : Female C57BL/6 mice were infected ocularly with virulent (McKrae) or avirulent (KOS, RE) strains of HSV-1. Virus titers in the eyes on days 3 and 5 post infection (PI), level of viral gB DNA in TG on day 28 PI, and virus reactivation on day 28 PI were measured.

Results : Our results suggest that the avirulent strains of HSV-1 even after corneal scarification had fewer viruses in the eye, less latency, and a longer time to reactivate than virulent strains of HSV-1. The time to explant reactivation of avirulent strains of HSV-1 was similar to that of the virulent LAT-minus McKrae derived mutant. The viral dose with the McKrae strains of HSV-1 affected level of viral DNA and time to explant reactivation.

Conclusions : Our results point to the absence of any correlation between the level of primary virus replication with the level of viral DNA during latency and neither were an indicator of how rapidly the virus reactivated following explant TG induced reactivation.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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