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Eduardo Sebastian Arellano Arias, Atzin Robles-Contreras; Differential expression of Tumoral Necrosis Factor Receptor in different types of cataract.. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2512.
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to compare the presence of the Tumoral Necrosis Factor receptor (TNFR) in lenses with senile, traumatic and diabetic cataract.
Lenses with clinical diagnosis of senile, traumatic and diabetic cataract were used to run the study. Material was homogenized and processed to quantify the total amount proteins. Dilutions were made in order to obtain the same amount of total proteins in each sample and ELISA was used for TNFRSF1A quantification. To analyze the data distribution and for group comparison the D’Agostino & Pearson and the Kruskall Wallis test were used respectively.
An average concentration of TNRF of 172.7±124.5 pg/ml in senile cataracts was found. In traumatic cataracts, the TNFR average was 409.8±124.5pg/ml and in diabetic cataracts 457.9±212.1 pg/ml. A statistically significant difference between senile and traumatic cataract (p=0.0051) and between senile and diabetic cataract (p=0.0067) was found. There was no statistical difference between traumatic and diabetic cataract (p=0.454).
Differential expression in TNFR levels was found in senile cataracts as compared with traumatic and diabetic cataracts. This overexpression in TNFR could be associated with increased levels of TNF which would imply an inflammatory mechanism in the development of traumatic and diabetic cataracts. This could also imply a role of apoptosis in the events that lead to the development of this pathology.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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