September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Selective mitochondrial autophagy in retinal ganglion cells following short-term reduction in cerebrospinal fluid pressure
Author Affiliations & Notes
  • li xiaoxia
    ophthalmology, Beijing Institute of Ophthalmology, Beijing, Beijing, China
    Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing, China
  • Zheng Zhang
    ophthalmology, Beijing Institute of Ophthalmology, Beijing, Beijing, China
    Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing, China
  • Qian Liu
    Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing, China
  • Ningli Wang
    ophthalmology, Beijing Institute of Ophthalmology, Beijing, Beijing, China
    Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing, China
  • Footnotes
    Commercial Relationships   li xiaoxia, None; Zheng Zhang, None; Qian Liu, None; Ningli Wang, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2535. doi:
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    • Get Citation

      li xiaoxia, Zheng Zhang, Qian Liu, Ningli Wang; Selective mitochondrial autophagy in retinal ganglion cells following short-term reduction in cerebrospinal fluid pressure. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2535.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Purpose : Short-term lowering of reduction in cerebrospinal fluid pressure(CSFP) with a disturbance of both the orthograde and retrograde axonal transport. To explore what underlie the axonal transport deficit, we examined mitochondria in retinal ganglion cells in Sprague Dawley rat.

Methods : In experimental group the CSFP was reduced by continuous aspiration of cerebrospinal fluid, and a sham control group (with a trocar in cisterna magna without cerebrospinal fluid release) was set. Under electron microscopy autophagosomes were observed in retinal ganglion cells. Selective mitochondrial autophagy related cytokines in retinal ganglion cells(RGCs) were analysis by immunohistochemistry or immunofluorescence. And protein was isolated from retinas for analysis of LC3-I,LC3-II, and lysosome-associated membrane protein 1 (LAMP1) by Western blot.

Results : The antophagosomes in ganglion cell were significantly increased, the autophagy related proteins LC3-I, LC3-II, LAMP1 were increased in retinal sections, and the protein expression changes were also certified by western blot in experimental group.

Conclusions : This present study showed that selective mitochondrial autophagy is activated in RGCs, which could be explain the disturbance of both the orthograde and retrograde axonal transport following short-term reduction in cerebrospinal fluid pressure.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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