September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Glutamine synthetase shift involved in protective effects of GFAP antibody on retinal ganglion cells
Author Affiliations & Notes
  • Katharina Bell
    Department of Ophthalmology, Experimental Ophthalmology, Mainz, Germany
  • Corina Wilding
    Department of Ophthalmology, Experimental Ophthalmology, Mainz, Germany
  • Jana Holz-Müller
    Department of Ophthalmology, Experimental Ophthalmology, Mainz, Germany
  • Sebastian Funke
    Department of Ophthalmology, Experimental Ophthalmology, Mainz, Germany
  • Norbert Pfeiffer
    Department of Ophthalmology, Experimental Ophthalmology, Mainz, Germany
  • Franz H Grus
    Department of Ophthalmology, Experimental Ophthalmology, Mainz, Germany
  • Footnotes
    Commercial Relationships   Katharina Bell, None; Corina Wilding, None; Jana Holz-Müller, None; Sebastian Funke, None; Norbert Pfeiffer, None; Franz Grus, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2567. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Katharina Bell, Corina Wilding, Jana Holz-Müller, Sebastian Funke, Norbert Pfeiffer, Franz H Grus; Glutamine synthetase shift involved in protective effects of GFAP antibody on retinal ganglion cells. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2567.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Glaucoma is a multifactorial, neurodegenerative disease. In the past up- as well as down-regulations were detected in the autoantibody profile in the serum of glaucoma patients. Previous studies were able to demonstrate protective effects of the down-regulated antibody against GFAP on a neuroretinal cell line. With this study we wanted to analyse the protective antibody effect in more detail using a porcine retinal organ culture.

Methods : The retina along with the RPE was isolated from porcine eyes derived from 3-6 month old pigs. The retinal explants were incubated either with 1 µg/ml GFAP antibodies for 24 h or without antibodies as control. Mass spectrometric analysis of the retinae were conducted. Following incubation Brn3a, DAPI, TUNEL and glutamine synthetase staining were performed. A quantification of retinal ganglion cells was performed as well as an analysis of the glutamine synthetase intensity. Glutamine synthetase analysis was performed by analyzing the overall staining in the retinae as well as the intensity of staining in the retinal ganglion cell layer.

Results : Rgc/mm increased significantly in GFAP ab incubated retinal explants (30 % more RGC/mm) (17.9 rgc/mm) (p=0.04) in comparison to the untreated controls (13.8 rgc/mm). Mass spectrometric analysis revealed elevated glutamine synthetase levels (2.5 fold upregulated in the GFAP ab incubated retinae). The overall intensity of glutamine synthetase in the retinae was slightly elevated in the GFAP group. When then analyzing the glutamine synthetase staining of the inner boarder of the retinae, significant differences between the ab incubated retinae and the control retinae (Intensity control retinae: 58.05; GFAP ab treated retinae: 85.96) (p< 0.05) were detected.

Conclusions : Low concentrations of GFAP antibodies have a protective effect on retinal ganglion cells. We conclude that there is a strong Müller cell participation in the demonstrated protective ab effects leading to a shift of glutamine synthetase towards the inner border of the retina. This could be either triggered via direct antibody/müller cell contact or indirectly via rgc/müller cell contact.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×