September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Histopathological Resource for AMD, Glaucoma and DIabetes - application to study of interphotoreceptor matrix
Author Affiliations & Notes
  • Federico Gonzalez-Fernandez
    Ophthalmology and Pathology, University of Mississippi Medical Center, Jackson, Mississippi, United States
    Research & Development, G.V. (Sonny) Montgomery Veterans Affairs Med Cente, Jackson, Mississippi, United States
  • Saboor Shad
    Fulfillment, National Disease Research Interchange, Philadelphia, Pennsylvania, United States
  • Gene Kopen
    Operations, National Disease Research Interchange, Philadephia, Pennsylvania, United States
  • Tianle Zou
    Ophthalmology and Pathology, SUNY at Buffalo , Buffalo, Mississippi, United States
  • Footnotes
    Commercial Relationships   Federico Gonzalez-Fernandez, None; Saboor Shad, None; Gene Kopen, None; Tianle Zou, None
  • Footnotes
    Support  Merit Review Award I01BX007080 from the Biomedical Laboratory Research & Development Service of the Veterans Affairs Office of Research and Development, NIH RO1 EY09412; National Center for Research Resources 5G12RR013646-12, National Institutes of Minority Health and Health Disparities (G12MD007591), an Unrestricted Research Grant from Research to Prevent Blindness to the Department of Ophthalmology at SUNY at Buffalo, and NIH grant 5 U42 RR006042 to the NDRI.
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2728. doi:
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    • Get Citation

      Federico Gonzalez-Fernandez, Saboor Shad, Gene Kopen, Tianle Zou; Histopathological Resource for AMD, Glaucoma and DIabetes - application to study of interphotoreceptor matrix. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2728.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Obtaining histological sections of globes with AMD, diabetes and glaucoma is difficult because eyes available through hospital pathology units typically represent end stage disease, and autopsy globes have significant autolysis. We sought to create an archive of histological material for the vision researchers. To illustrate the usefulness of the collection, a characterization of the pathology of the interphotoreceptor matrix (IPM).

Methods : Globes were obtained from various eye banks for Donors with diabetes, AMD, glaucoma and normal controls. Clinical history was correlated with detailed gross and histopathological study and serial sections prepared through the fovea. Immunohistochemistry compared the distribution of IRBP and peanut agglutinin binding matrix domains, with that of the choroidal proteins albumin, and BIGH3. Globes were retrieved from 32 donors (30 to 98 yrs) with diabetes, AMD, glaucoma, and controls. The interval between death and placement of the eye in formalin was under 10.5 hrs except in one control. Cases: Diabetes (2 no apparent diabetic retinopathy, 4 non-proliferative, 2 proliferative); AMD (5 dry, 2 wet); glaucoma (11 cases), and normal controls (6 cases). Nine had more than one diagnosis.

Results : Except when the postmortem interval was beyond 12 hrs, IRBP was restricted to the IPM; albumin and BIGH3 to the choroid. In diabetes, IRBP was reduced in the IPM, and albumin was often present. No redistribution was seen in glaucoma. Dry AMD was associated with entry of IRBP between the RPE and Bruch’s membrane; In wet AMD there was reduced expression of IRBP in the IPM and appearance of IRBP in the choroid and BIGH3 in the disciform scar.

Conclusions : An archive of the various stages of AMD, glaucoma and diabetes was established. Careful correlation of clinical history with gross and histopathological findings was critical to accurately characterizing the disease type and stage. Often inaccurate clinical history had to be corrected with the the pathology results. Furthermore, conditions not recognized clinically were often uncovered. Our data suggests that changes in the IPM may be relevant to the pathophysiology of diabetes and AMD. The histopathology archive, which is available to Vision Researchers, will be useful to study the expression and distribution of new markers in these difficult to obtain ocular specimens.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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