September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
AMPK activation protects photoreceptors from light-induced degeneration.
Author Affiliations & Notes
  • Hirohiko Kawashima
    Public Health/Ophthalmology, Keio University Hospital, Shinnjuku-ku, Japan
  • Hideto Osada
    Public Health/Ophthalmology, Keio University Hospital, Shinnjuku-ku, Japan
  • Eriko Toda
    Public Health/Ophthalmology, Keio University Hospital, Shinnjuku-ku, Japan
  • Tomohiro Okamoto
    Public Health/Ophthalmology, Keio University Hospital, Shinnjuku-ku, Japan
  • Mamoru Kamoshita
    Public Health/Ophthalmology, Keio University Hospital, Shinnjuku-ku, Japan
  • Norihiro Nagai
    Public Health/Ophthalmology, Keio University Hospital, Shinnjuku-ku, Japan
  • Kazuo Tsubota
    Public Health/Ophthalmology, Keio University Hospital, Shinnjuku-ku, Japan
  • Yoko Ozawa
    Public Health/Ophthalmology, Keio University Hospital, Shinnjuku-ku, Japan
  • Footnotes
    Commercial Relationships   Hirohiko Kawashima, None; Hideto Osada, None; Eriko Toda, None; Tomohiro Okamoto, None; Mamoru Kamoshita, None; Norihiro Nagai, None; Kazuo Tsubota, None; Yoko Ozawa, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2734. doi:
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      Hirohiko Kawashima, Hideto Osada, Eriko Toda, Tomohiro Okamoto, Mamoru Kamoshita, Norihiro Nagai, Kazuo Tsubota, Yoko Ozawa; AMPK activation protects photoreceptors from light-induced degeneration.. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2734.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To elucidate the neuroprotective effect of activated AMP-activated protein kinase (AMPK) in the light exposed retina, focusing on the light-induced photoreceptor degeneration.

Methods : Seven- to 8-week-old BALB/c mice were divided into 4 groups and treated either with control vehicle or an AMPK activator, 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR) at 125 or 250 or 500 mg/kg body weight, twice, before and after 12 hours of dark adaptation. The mice were then, exposed to a white fluorescent lamp for 1 hour at 3000 lux. Two days after light exposure, full-field scotopic electroretinogram was recorded in response to the flash light stimuli at intensities ranging from −2.12 to 2.89 log cd s/m2, and rhodopsin protein was measured in the retinal sample by immunoblot analysis.

Results : The amplitudes of a-waves were larger in the AICAR treated group in a dose dependent manner; the mice treated with either 250 or 500 mg/kg BW of AICAR showed significantly larger a-wave amplitudes. Along with this, b-wave amplitudes were also larger in these group under some of the stimulus condition. There were no changes in the implicit times of a-wave and b-wave. Significantly larger amount of rhodopsin protein was observed after light exposure when treated with 500 mg/kg BW of AICAR.

Conclusions : Although further studies are required, AMPK activation may have a neuroprotective effect against light-induced photoreceptor degeneration.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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