September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
The effect of 3% diquafosol sodium eye drops on the meibomian gland alterations of the superoxide dismutase-1 knockout mice
Author Affiliations & Notes
  • Keisuke Ikeda
    Keio University School of Medicine, Tokyo, Japan
    Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Ichikawa, Japan
  • Murat Dogru
    Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Ichikawa, Japan
    Keio University School of Medicine, Tokyo, Japan
  • Motoko Kawashima
    Keio University School of Medicine, Tokyo, Japan
  • Takashi Kojima
    Keio University School of Medicine, Tokyo, Japan
  • Kazunari Higa
    Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Ichikawa, Japan
  • Ayako Igarashi
    Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Ichikawa, Japan
  • Takefumi Yamaguchi
    Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Ichikawa, Japan
  • Seika Den-Shimazaki
    Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Ichikawa, Japan
  • Kazuo Tsubota
    Keio University School of Medicine, Tokyo, Japan
  • Jun Shimazaki
    Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Ichikawa, Japan
  • Footnotes
    Commercial Relationships   Keisuke Ikeda, Santen (F); Murat Dogru, Santen (F); Motoko Kawashima, None; Takashi Kojima, None; Kazunari Higa, Santen (F); Ayako Igarashi, None; Takefumi Yamaguchi, None; Seika Den-Shimazaki, None; Kazuo Tsubota, None; Jun Shimazaki, Santen (F)
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2869. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Keisuke Ikeda, Murat Dogru, Motoko Kawashima, Takashi Kojima, Kazunari Higa, Ayako Igarashi, Takefumi Yamaguchi, Seika Den-Shimazaki, Kazuo Tsubota, Jun Shimazaki; The effect of 3% diquafosol sodium eye drops on the meibomian gland alterations of the superoxide dismutase-1 knockout mice. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2869.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Superoxide dismutase-1 knock out (SOD-1 KO) mouse has been reported to be a model for age related meibomian gland(MG) alterations. We investigated the effect of a two week 3% diquafosol sodium (DQS) eye drop application (6 times/day) on the MGs in the SOD-1 KO mice in comparison to the wild type mice.

Methods : Ten eyes of 5 SOD-1 KO male mice with C57BL/background and 10 eyes of 5 C57BL6 strain wild–type male mice were examined at 50 weeks before and 2 weeks after application of 3 % DQS eye drops. Tear film stability and corneal epithelial damage was evaluated by fluorescein and lissamine green (LG) stainings. Anterior segment photography was carried out before and after eye drop instillations. Aqueous tear quantity was measured with phenol-red-impregnated cotton threads without anesthesia. Animals were sacrificed after eye drop application at 52 weeks and the ocular specimens underwent HE, Oil red O as well as cytokeratin 4 and 13 immunohistochemistry stainings. Quantitative real time-PCR for cytokeratin 4 and 13 mRNA expression was also performed. All studies were performed in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.

Results : The mean pre-treatment fluorescein and LG staining scores were significantly higher and the mean BUT was significantly shorter in the SOD-1 KO mice compared to the WT mice. These scores improved significantly in the SOD-1 KO mice after 2 weeks of DQS treatment (p<0.01). The cytokeratin 4 and 13 staining intensity and the mRNA expressions decreased significantly with 3% DQS treatment (p<0.01). The mean number of secretory lipid droplets in the meibomian glands as quantified with Oil red O staining increased significantly after DQS treatment (p<0.01).

Conclusions : Two week application of 3% DQS eye drops was found to be effective in terms of improvement of meibomian gland keratinization and lipid secretion in the SOD-1 KO mice.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×