September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Vasodilating effect of Ripasudil Hydrochloride Hydrate on isolated rabbit posterior ciliary arteries
Author Affiliations & Notes
  • Takako Sugimoto
    Opthalmology, Miyazaki University, Miyazaki, Miyazaki, Japan
  • Hideki Chuman
    Opthalmology, Miyazaki University, Miyazaki, Miyazaki, Japan
  • Nobuhisa Nao-i
    Opthalmology, Miyazaki University, Miyazaki, Miyazaki, Japan
  • Footnotes
    Commercial Relationships   Takako Sugimoto, None; Hideki Chuman, None; Nobuhisa Nao-i, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2998. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Takako Sugimoto, Hideki Chuman, Nobuhisa Nao-i; Vasodilating effect of Ripasudil Hydrochloride Hydrate on isolated rabbit posterior ciliary arteries. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2998.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Ripasudil (K-115) is a Rho-kinase inhibitor and is an ocular hypotensive agent. Ripasudil also has a vasodilating effect. The optic disc blood flow is supplied by posterior ciliary arteries (PCAs). This study aimed to clarify the vasodilatory effect of exogenous Ripasudil on isolated PCAs.

Methods : Vascular ring segments were mounted on a double myograph system. After obtaining the maximal contraction following the administration of a high-K solution, different concentrations of Ripasudil (100 nM to 100 μM) were administrated. When a vasodilatory effect was observed, carboxy-2-phenyl-4,4,5,5,-tetramethyl-imidazoline-1-oxyl-3-oxide (carboxy-PTIO), a nitric oxide scavenger, or NG-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, were administered. All isometric force measurements are given as relative values compared with high-K–induced maximal contractions.

Results : Ripasudil significantly relaxed high-K solution-induced contracted rabbit PCAs in a concentration dependent manner (100 nM [34.8%±3.5]; 1 μM [78.5±17.3]; 10 μM [91.68±9.0]; 100 μM [94.41±8.3]). Carboxy-PTIO (1 mM) or L-NAME (300 mM) did not inhibit Ripasudil-induced relaxation in rabbit PCAs.

Conclusions : Ripasudil has a nitric oxide independent vasodilatory effect on high K-induced contractions in isolated rabbit PCAs.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×