September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
RanGAP1, the most Prominently SUMOylated Lens Proteins Regulates Cell Cycle in Lens
Author Affiliations & Notes
  • David W Li
    University of Nebraska Medical Center, Omaha, Nebraska, United States
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong Province, China
  • Weike Ji
    University of Nebraska Medical Center, Omaha, Nebraska, United States
  • Xiaohui Hu
    University of Nebraska Medical Center, Omaha, Nebraska, United States
  • Lili Gong
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong Province, China
  • Zhaoxia Huang
    University of Nebraska Medical Center, Omaha, Nebraska, United States
  • Ling Wang
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong Province, China
    University of Nebraska Medical Center, Omaha, Nebraska, United States
  • Yizhi Liu
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong Province, China
  • Quan Dong Nguyen
    University of Nebraska Medical Center, Omaha, Nebraska, United States
  • Footnotes
    Commercial Relationships   David Li, None; Weike Ji, None; Xiaohui Hu, None; Lili Gong, None; Zhaoxia Huang, None; Ling Wang, None; Yizhi Liu, None; Quan Dong Nguyen, None
  • Footnotes
    Support  Research to Prevent Blindness, NSFC, Zhongshan Ophthalmic Center, CHinese Scholarship Council
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3068. doi:
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      David W Li, Weike Ji, Xiaohui Hu, Lili Gong, Zhaoxia Huang, Ling Wang, Yizhi Liu, Quan Dong Nguyen; RanGAP1, the most Prominently SUMOylated Lens Proteins Regulates Cell Cycle in Lens
      . Invest. Ophthalmol. Vis. Sci. 2016;57(12):3068.

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      © 2017 Association for Research in Vision and Ophthalmology.

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Abstract

Purpose : SUMOylation is an important post-translation modification to regulate protein functions. Our previous studies have shown that SUMOylation activates the p32 Pax6 to regulate downstream gene expression both in vitro and in vivo (Yan et al., 1990. PNAS). Moreover, our recent studies have shown that SUMOylation regulates SP-1 to account for differential regulation of lens differentiation (Gong et al. 2014. PNAS). Here we demonstrate that SUMOylation of the most prominent SUMO-conjugated lens protein regulates cell cycle of lens epithelial cells and plays an important role during lens development.

Methods : Confocal microscope was used to record the cell cycle progress. Immunocytochemistry was used to detect the expression and localization of RanGAP1 and related protein components. SiRNA-mediated knockdown of RanGAP1 and pRedGFP-RanGAP1-mediated over expression were used to change the expression levels of RanGAP1.

Results : RanGAP1 expression was knocked down in mouse lens epithelial cells (aTN4-1) and human lens epithelial cells (HLE) via siRNA or shRNA respectively, and consequently both two types of lens cells were shown significantly abnormality in their cell cycle progression. RanGap1 knockdown resulted in mitotic spindle defects, leading to chromosome misalignment with abnormal cytokinesis and multinuclear cells.

Conclusions : SUMOylation regulation of RanGAP1 plays an important role in helping to execute its normal functions in cell cycle control. (Supported by Research prevent blindness, NSFC and Zhongshan Ophthalmic Center)

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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