September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Rho/ROCK-mediated Retinal Endothelial Stiffening Impairs TRPV4 Signaling and Promotes Diabetic Retinal Inflammation
Author Affiliations & Notes
  • Xiao Yang
    Bioengineering, University of California, Riverside, Riverside, California, United States
  • Arun Bhaskaran
    Bioengineering, University of California, Riverside, Riverside, California, United States
  • Harry Scott
    Bioengineering, University of California, Riverside, Riverside, California, United States
  • Soroush Ardekani
    Bioengineering, University of California, Riverside, Riverside, California, United States
  • Jun Xu
    Physic, University of California, Riverside, Riverside, California, United States
  • Umar Mohideen
    Physic, University of California, Riverside, Riverside, California, United States
  • Timothy S Kern
    Case Western Reserve University, Cleveland, Ohio, United States
  • Kaustabh Ghosh
    Bioengineering, University of California, Riverside, Riverside, California, United States
  • Footnotes
    Commercial Relationships   Xiao Yang, None; Arun Bhaskaran, None; Harry Scott, None; Soroush Ardekani, None; Jun Xu, None; Umar Mohideen, None; Timothy Kern, None; Kaustabh Ghosh, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3220. doi:
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    • Get Citation

      Xiao Yang, Arun Bhaskaran, Harry Scott, Soroush Ardekani, Jun Xu, Umar Mohideen, Timothy S Kern, Kaustabh Ghosh; Rho/ROCK-mediated Retinal Endothelial Stiffening Impairs TRPV4 Signaling and Promotes Diabetic Retinal Inflammation. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3220.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Retinal inflammation is a hallmark of diabetic retinopathy (DR). We have recently shown that loss of mechanosensitive ion channel Transient Receptor Potential Vanilloid 4 (TRPV4) activity contributes to hyperglycemia-induced retinal endothelial cell (EC) activation, a critical early event in diabetic retinal inflammation. However, the mechanotransduction pathway involved in TRPV4-mediated retinal EC activation remains unknown. Since Rho is a key molecular player in mechanotransduction and increased Rho/ROCK promotes EC activation by suppressing endothelial nitric oxide (NO) production, we here examined the role of Rho/ROCK in TRPV4-mediated diabetic retinal EC activation.

Methods : Retinal capillaries were isolated from control and streptozotocin-induced diabetic mice for detection of ROCK expression by Western Blot (WB). Human retinal EC cultures were treated with normal glucose (5.5 mM) or high glucose (HG; 30 mM) for 10d and Rho activity was assessed by G-LISA assay. Stiffness of isolated mouse retinal capillaries and cultured human ECs, which is directly regulated by Rho/ROCK, was measured by atomic force microscopy. EC activation was assessed by measuring the levels of intracellular NO, ICAM-1 expression, and monocyte-EC adhesion. Expression and activity of TRPV4 was determined by WB and Ca2+ microfluorimetry, respectively. TRPV4 and ROCK activities were modulated using selective pharmacological antagonist and agonist.

Results : Diabetic mouse retinal capillaries exhibited significantly greater ROCK expression and stiffness, which correlated with Rho/ROCK activation and stiffening of HG-treated human retinal ECs. The HG-treated stiffer ECs underwent significant activation, which was consistent with a marked decrease in expression and activity of TRPV4. Importantly, inhibition of ROCK activity alone caused significant recovery of TRPV4 expression/activity, endothelial NO production and suppression of monocyte-EC adhesion. Conversely, pharmacological enhancement of TRPV4 suppressed Rho-mediated EC stiffening and the inflammatory effects in vitro.

Conclusions : These findings indicate that Rho/ROCK acts as both an effector and target of TRPV4 and implicate Rho/ROCK/TRPV4 axis as a crucial regulator of diabetic retinal inflammation associated with DR.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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